Genetic diversity analyse of Thailand Babylonia areolata with RAPD markers
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Graphical Abstract
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Abstract
Random Amplified Polymorphic DNA(RAPD)technique was applied to detect DNA polymorphism in 20 individuals of an artificially grown population of Babylonia areolata imported from Thailand.21 random primers were used from which a total of 222 bands were generated.Each primer used in the detection has 4~16 bands,with DNA fragment lengths ranging from 200 to 2200bp.Observed total amount and percentage of the polymorphic fragments are 156 and70.27%,and the Shannon and Nei index of phenotypic and gene diversity of raised population are 0.2818 and 0.2491 respectively.Genetic distance among individuals ranges from 0.066 to 0.291.Comparing genetic diversity of B.areolata with that of other shellfish reveals a hefty genetic diversity of B.areolata.
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