Population differentiation of wild soybean based on the RAPD and SSR analysis

Random amplified polymorphic DNA (RAPD)and simple sequence repeat(SSR)analyses were applied to 16 samples of wild soybeans(Glycine soja sieb and Zucc)originated from the region of 25^。N．Twenty primers screened with 150 ones yielded 146 RAPD bands which had 60 polymorphic products and 40．8％ of total bands．the average heterozygosity(He)was 0．3248 and the average genetic distance among the 16 samples was 0．1536．Fourteen pairs of soybean SSR primers screened had polymorphism ．the average heterozygosity was 0．6961 and the average genetic distance was 0．2209．Cluster analysis showed there are five small classes divided from the 16 samples and there are some evidence showing genetic variations in the population．The genetic resource is also put forward．