Technology of tissue cultural propagation of Malus zumi

Culturing the stems of Malus zumi in vitro,the key techniques including medium,auxin,age of sprouts and agar are studied.The results show that if there is the advanced medium MS+6-BA 0.5mg/L+IAA0.2mg/L for multiplicating ，1/2MS+IAA0.5mg/L or 1/2MS+IBA0.5mg/L for rooting,sprout's age being 30～40 days,agar corresponding to the international standard and the light intensity sustaining at 1500～2000lx,the coefficient of multiplication,the quantity of effictive plantlets and the rate of rootage and growth are best,and the surviving ratio of transplantation of plantlets and nursery seedling is above 90% and 85% respectively,and 1-yr-old tree height is above 1.5m.Based on the study,the industrializing technology of tube-plantlets of Malus zumi and the culture standard which improves a theoretical basis for vegetive propagation of others plant are summarized.