A preliminary study on the technology for storage of tissue-cultured plantlets in low temperature condition

After stored in natural low temperature(0～10℃)，forest tree tissue-cultured plantlets had a higher survival rate, their chilling resistant ability was increased visibly. The results from observing and analysing the morphological and physiological characters of the platlets at different storage period showed that with the decreasing of storage temperature,starch content was decreased, while the soluble sugar content increased,crude protein content was increased slightly with fluctuation,which are the physiological reactions that the tissue-cultured plantlets adapted to low temperature and weak light.The losing rates after storage for the 165 days differented plantlets and 120 days rooted plantlets were all lower than 10.0%.After the storage the effective number of the differented plantlets was greatly increased with more than 95.5% rooting rate and the transplanting survival rate of the rooted plantlet reached 93.5%～98.5%.