Effects of straw returning and fertilization on soil bacterial and fungal community structures and diversities in rice-wheat rotation soil

ZHANG Hanlin; BAI Naling; ZHENG Xianqing; LI Shuangxi; ZHANG Juanqin; ZHANG Haiyun; ZHOU Sheng; SUN Huifeng; LYU Weiguang

doi:10.13930/j.cnki.cjea.200502

Volume 29 Issue 3

Mar. 2021

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Article Navigation > Chinese Journal of Eco-Agriculture > 2021 > 29(3): 531-539

ZHANG Hanlin, BAI Naling, ZHENG Xianqing, LI Shuangxi, ZHANG Juanqin, ZHANG Haiyun, ZHOU Sheng, SUN Huifeng, LYU Weiguang. Effects of straw returning and fertilization on soil bacterial and fungal community structures and diversities in rice-wheat rotation soil[J]. Chinese Journal of Eco-Agriculture, 2021, 29(3): 531-539. doi: 10.13930/j.cnki.cjea.200502

Citation:

ZHANG Hanlin, BAI Naling, ZHENG Xianqing, LI Shuangxi, ZHANG Juanqin, ZHANG Haiyun, ZHOU Sheng, SUN Huifeng, LYU Weiguang. Effects of straw returning and fertilization on soil bacterial and fungal community structures and diversities in rice-wheat rotation soil[J]. Chinese Journal of Eco-Agriculture, 2021, 29(3): 531-539. doi: 10.13930/j.cnki.cjea.200502

Citation:

ZHANG Hanlin, BAI Naling, ZHENG Xianqing, LI Shuangxi, ZHANG Juanqin, ZHANG Haiyun, ZHOU Sheng, SUN Huifeng, LYU Weiguang. Effects of straw returning and fertilization on soil bacterial and fungal community structures and diversities in rice-wheat rotation soil[J]. Chinese Journal of Eco-Agriculture, 2021, 29(3): 531-539. doi: 10.13930/j.cnki.cjea.200502

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Effects of straw returning and fertilization on soil bacterial and fungal community structures and diversities in rice-wheat rotation soil

doi: 10.13930/j.cnki.cjea.200502

ZHANG Hanlin^{1, 2
,},
BAI Naling^{1, 2},
ZHENG Xianqing^{1, 2},
LI Shuangxi^{1, 2},
ZHANG Juanqin^{1, 2},
ZHANG Haiyun^{1, 2},
ZHOU Sheng^{1, 3},
SUN Huifeng^{1, 3},
LYU Weiguang^{1, 2
,
,}

1.
Eco-environmental Protection Institute of Shanghai Academy of Agricultural Sciences/Shanghai Key Laboratory of Horticultural Technology/Environmental Protection Monitoring Station of Shanghai City, Shanghai 201403, China
2.
Agricultural Environment and Farmland Conservation Experiment Station of Ministry Agriculture, Shanghai 201403, China
3.
Shanghai Low Carbon Agriculture Engineering Technology Research Center, Shanghai 201403, China

Funds:

the National Key Research and Development Program of China 2016YFD0200804

the Shanghai Agriculture Applied Technology Development Program, China T20180414

the Outstanding Team Program of Shanghai Academy of Agricultural Sciences Nongke Chuang 2017(A-03)

More Information

Corresponding author: LYU Weiguang, E-mail: lvweiguang@saas.sh.cn
Received Date: 2020-06-26
Accepted Date: 2020-11-23
Publish Date: 2021-03-01

Abstract

Abstract

Straw returning and slow-release fertilizers are widely used in agriculture to reduce non-point source pollution and improve nitrogen use efficiency. However, there are few studies on the effect of straw return combined with slow-release fertilizers on the soil microbial community. This study determined how straw returning combined with fertilization affected the bacterial and fungal communities in rice-wheat rotation soil and the underlying mechanisms. Based on a seven-year rice-wheat rotation system monitoring experiment, four treatments were selected: no fertilizer (CK), regular chemical fertilization (RT), straw returning combined with chemical fertilization (RS), and straw returning combined with slow-release fertilizer (SS). The Illumina MiSeq platform was used to evaluate the community structure and diversity of soil bacteria and fungi and to detect the primary environmental factors affecting the microbial community. The results showed that the SS rice and wheat yields in 2016 and 2017 were significantly higher than the RT yields (by 11.6% and 8.2% in rice, and 4.8% and 3.6% in wheat, respectively); there was no difference between SS and RS yields. Compared to RT, straw returning significantly decreased soil pH and increased soil organic carbon (SOC) and ammonium nitrogen (NH₄⁺-N) contents; SS had more NH₄⁺-N than RS. The fungal community diversity was higher with straw returning than with RT, but there was no difference in the bacterial community diversity among fertilization treatments; the bacterial and fungal community diversities were the same between RS and SS. Correlation analysis showed that the bacterial community diversity was negatively correlated with pH and positively correlated with soil total nitrogen content, while the fungal community diversity was positively correlated with SOC. Non-metric multidimensional scaling analysis showed that fertilization had a greater effect on the bacterial community structure (55.61%), and straw returning had a greater effect on the fungal community structure (26.94%). Proteobacteria, Chloroflexi, and Acidobacteria (in successive order) were the dominant phyla across all treatments, accounting for 66.07%–71.76% of the total bacterial sequence data. Ascomycota, Basidiomycota, and Zygomycota (in successive order) were the dominant phyla across all treatments, accounting for 88.05%–89.04% of the total fungal sequence data. Compared with RT, the treatments with straw returning significantly increased the relative abundance of Actinobacteria, Chloroflexi, and Firmicutes in the bacterial community, and significantly increased the relative abundance of Ascomycota and decreased the relative abundance of Basidiomycota and Zygomycota in the fungal community. Ecological function analysis of these bacterial and fungal communities showed that straw returning may enhance soil carbon and nitrogen cycling and inhibit pathogens. Compared with RS, SS only increased the relative abundance of Ascomycota in the fungal community; there were no other differences between the relative abundances of bacteria and fungi in RS and SS at the phylum level. Straw returning with slow-release fertilizers can help maintain or improve soil nutrient availability, crop yield, and the diversity of bacterial and fungal communities and can promote soil carbon and nitrogen cycling.
- Soil microorganism,
- Community structure,
- Soil nutrient,
- Straw returning,
- Slow release fertilizer,
- High throughput sequencing

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References(35)

References

[1]	LI X B, HE H B, ZHANG X D, et al. Distinct responses of soil fungal and bacterial nitrate immobilization to land conversion from forest to agriculture[J]. Soil Biology and Biochemistry, 2019, 134: 81-89 doi: 10.1016/j.soilbio.2019.03.023
[2]	HAMM A C, TENUTA M, KRAUSE D O, et al. Bacterial communities of an agricultural soil amended with solid pig and dairy manures, and urea fertilizer[J]. Applied Soil Ecology, 2016, 103: 61-71 doi: 10.1016/j.apsoil.2016.02.015
[3]	王小玲, 马琨, 伏云珍, 等. 免耕覆盖及有机肥施用对土壤真菌群落组成及多样性的影响[J]. 应用生态学报, 2020, 31(3): 890-898 https://www.cnki.com.cn/Article/CJFDTOTAL-YYSB202003024.htm WANG X L, MA K, FU Y Z, et al. Effects of no-tillage, mulching, and organic fertilization on soil fungal community composition and diversity[J]. Chinese Journal of Applied Ecology, 2020, 31(3): 890-898 https://www.cnki.com.cn/Article/CJFDTOTAL-YYSB202003024.htm
[4]	SILVA M C P E, SEMENOV A V, SCHMITT H, et al. Microbe-mediated processes as indicators to establish the normal operating range of soil functioning[J]. Soil Biology and Biochemistry, 2013, 57: 995-1002 doi: 10.1016/j.soilbio.2012.10.002
[5]	HOLLISTER E B, SCHADT C W, PALUMBO A V, et al. Structural and functional diversity of soil bacterial and fungal communities following woody plant encroachment in the southern Great Plains[J]. Soil Biology and Biochemistry, 2010, 42(10): 1816-1824 doi: 10.1016/j.soilbio.2010.06.022
[6]	逯非, 王效科, 韩冰, 等. 稻田秸秆还田: 土壤固碳与甲烷增排[J]. 应用生态学报, 2010, 21(1): 99-108 https://www.cnki.com.cn/Article/CJFDTOTAL-YYSB201001016.htm LU F, WANG X K, HAN B, et al. Straw return to rice paddy: Soil carbon sequestration and increased methane emission[J]. Chinese Journal of Applied Ecology, 2010, 21(1): 99-108 https://www.cnki.com.cn/Article/CJFDTOTAL-YYSB201001016.htm
[7]	郭梨锦, 曹凑贵, 张枝盛, 等. 耕作方式和秸秆还田对稻田表层土壤微生物群落的短期影响[J]. 农业环境科学学报, 2013, 32(8): 1577-1584 https://www.cnki.com.cn/Article/CJFDTOTAL-NHBH201308015.htm GUO L J, CAO C G, ZHANG Z S, et al. Short-term effects of tillage practices and wheat-straw returned to rice fields on topsoil microbial community structure and microbial diversity in central China[J]. Journal of Agro-Environment Science, 2013, 32(8): 1577-1584 https://www.cnki.com.cn/Article/CJFDTOTAL-NHBH201308015.htm
[8]	SUN R B, ZHANG X X, GUO X S, et al. Bacterial diversity in soils subjected to long-term chemical fertilization can be more stably maintained with the addition of livestock manure than wheat straw[J]. Soil Biology and Biochemistry, 2015, 88: 9-18 doi: 10.1016/j.soilbio.2015.05.007
[9]	CHEN Z M, WANG H Y, LIU X W, et al. Changes in soil microbial community and organic carbon fractions under short-term straw return in a rice-wheat cropping system[J]. Soil and Tillage Research, 2017, 165: 121-127 doi: 10.1016/j.still.2016.07.018
[10]	萨如拉, 杨恒山, 邰继承, 等. 秸秆还田条件下腐熟剂对不同质地土壤真菌多样性的影响[J]. 中国生态农业学报(中英文), 2020, 28(7): 1061-1071 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?file_no=2020-0713&flag=1 SA R L, YANG H S, TAI J C, et al. Effect of straw maturing agents on fungal diversity in soil with different textures soil under return straw conditions[J]. Chinese Journal of Eco-Agriculture, 2020, 28(7): 1061-1071 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?file_no=2020-0713&flag=1
[11]	YANG Y C, ZHANG M, LI Y C, et al. Controlled release urea improved nitrogen use efficiency, activities of leaf enzymes, and rice yield[J]. Soil Science Society of America Journal, 2012, 76(6): 2307-2317 doi: 10.2136/sssaj2012.0173
[12]	PAN P, JIANG H M, ZHANG J F, et al. Shifts in soil bacterial communities induced by the controlled-release fertilizer coatings[J]. Journal of Integrative Agriculture, 2016, 15(12): 2855-2864 doi: 10.1016/S2095-3119(15)61309-0
[13]	孙会峰, 周胜, 付子轼, 等. 秸秆与缓释肥配施对水稻产量及氮素吸收利用率的影响[J]. 中国稻米, 2015, 21(4): 95-98 https://www.cnki.com.cn/Article/CJFDTOTAL-DAOM201504021.htm SUN H F, ZHOU S, FU Z S, et al. Effects of application of controlled-release fertilizer combined with wheat straw on rice yield and nitrogen use efficiency[J]. China Rice, 2015, 21(4): 95-98 https://www.cnki.com.cn/Article/CJFDTOTAL-DAOM201504021.htm
[14]	中国科学院南京土壤研究所. 土壤理化分析[M]. 上海: 上海科学技术出版社, 1981: 62−142 Nanjing Institute of Soil Science, Chinese Academy of Sciences. Soil Physical and Chemical Property Analysis[M]. Shanghai: Shanghai Scientific and Technical Publishers, 1981: 62−142
[15]	ZHOU J Z, WU L Y, DENG Y, et al. Reproducibility and quantitation of amplicon sequencing-based detection[J]. The ISME Journal, 2011, 5(8): 1303-1313 doi: 10.1038/ismej.2011.11
[16]	SCHOCH C L, SEIFERT K A, HUHNDORF S, et al. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(16): 6241-6246 doi: 10.1073/pnas.1117018109
[17]	HU X J, LIU J J, WEI D, et al. Soil bacterial communities under different long-term fertilization regimes in three locations across the black soil region of Northeast China[J]. Pedosphere, 2018, 28(5): 751-763 doi: 10.1016/S1002-0160(18)60040-2
[18]	ZHU J, PENG H, JI X H, et al. Effects of reduced inorganic fertilization and rice straw recovery on soil enzyme activities and bacterial community in double-rice paddy soils[J]. European Journal of Soil Biology, 2019, 94: 103116 doi: 10.1016/j.ejsobi.2019.103116
[19]	ABUJABHAH I S, BOUND S A, DOYLE R, et al. Effects of biochar and compost amendments on soil physico- chemical properties and the total community within a temperate agricultural soil[J]. Applied Soil Ecology, 2016, 98: 243-253 doi: 10.1016/j.apsoil.2015.10.021
[20]	WU Y P, WU J X, MA Y J, et al. Dynamic changes in soil chemical properties and microbial community structure in response to different nitrogen fertilizers in an acidified celery soil[J]. Soil Ecology Letters, 2019, 1(3/4): 105-113 doi: 10.1007/s42832-019-0012-z
[21]	张鑫, 周卫, 艾超, 等. 秸秆还田下氮肥运筹对夏玉米不同时期土壤酶活性及细菌群落结构的影响[J]. 植物营养与肥料学报, 2020, 26(2): 295-306 https://www.cnki.com.cn/Article/CJFDTOTAL-ZWYF202002010.htm ZHANG X, ZHOU W, AI C, et al. Effects of nitrogen management on soil enzyme activities and bacterial community structure in summer maize growing stagesunder straw incorporation[J]. Journal of Plant Nutrition and Fertilizers, 2020, 26(2): 295-306 https://www.cnki.com.cn/Article/CJFDTOTAL-ZWYF202002010.htm
[22]	周阳, 黄旭, 赵海燕, 等. 麦秸秆和沼液配施对水稻苗期生长和土壤微生物的调控[J]. 土壤学报, 2020, 57(2): 479-489 https://www.cnki.com.cn/Article/CJFDTOTAL-TRXB202002021.htm ZHOU Y, HUANG X, ZHAO H Y, et al. Regulation of wheat straw and biogas slurry application on rice seedling growth and soil microorganism[J]. Acta Pedologica Sinica, 2020, 57(2): 479-489 https://www.cnki.com.cn/Article/CJFDTOTAL-TRXB202002021.htm
[23]	张鹏, 贾志宽, 王维, 等. 秸秆还田对宁南半干旱地区土壤团聚体特征的影响[J]. 中国农业科学, 2012, 45(8): 1513-1520 doi: 10.3864/j.issn.0578-1752.2012.08.007 ZHANG P, JIA Z K, WANG W, et al. Effects of straw returning on characteristics of soil aggregates in semi-arid areas in southern Ningxia of China[J]. Scientia Agricultura Sinica, 2012, 45(8): 1513-1520 doi: 10.3864/j.issn.0578-1752.2012.08.007
[24]	KAISER K, WEMHEUER B, KOROLKOW V, et al. Driving forces of soil bacterial community structure, diversity, and function in temperate grasslands and forests[J]. Scientific Reports, 2016, 6: 33696 doi: 10.1038/srep33696
[25]	何敏红, 何跃军, 吴春玉, 等. 石漠化强度对喀斯特植被演替过程土壤真菌组成及多样性的影响[J]. 菌物学报, 2019, 38(4): 471-484 https://www.cnki.com.cn/Article/CJFDTOTAL-JWXT201904002.htm HE M H, HE Y J, WU C Y, et al. Effects of rocky desertification intensity on soil fungal composition and diversity during karst vegetation succession[J]. Mycosystema, 2019, 38(4): 471-484 https://www.cnki.com.cn/Article/CJFDTOTAL-JWXT201904002.htm
[26]	FAN F L, YIN C, TANG Y J, et al. Probing potential microbial coupling of carbon and nitrogen cycling during decomposition of maize residue by ¹³C-DNA-SIP[J]. Soil Biology and Biochemistry, 2014, 70: 12-21 doi: 10.1016/j.soilbio.2013.12.002
[27]	TRIVEDI P, ANDERSON I C, SINGH B K. Microbial modulators of soil carbon storage: integrating genomic and metabolic knowledge for global prediction[J]. Trends in Microbiology, 2013, 21(12): 641-651 doi: 10.1016/j.tim.2013.09.005
[28]	XU M, XIAN Y, WU J, et al. Effect of biogas slurry addition on soil properties, yields, and bacterial composition in the rice-rape rotation ecosystem over 3 years[J]. Journal of Soils and Sediments, 2019, 19(5): 2534-2542 https://www.zhangqiaokeyan.com/academic-journal-foreign_other_thesis/0204113063302.html
[29]	ZHAO S C, QIU S J, XU X P, et al. Change in straw decomposition rate and soil microbial community composition after straw addition in different long-term fertilization soils[J]. Applied Soil Ecology, 2019, 138: 123-133 http://www.sciencedirect.com/science/article/pii/S092913931830876X
[30]	SOUZA R C, MENDES I C, REIS-JUNIOR F B, et al. Shifts in taxonomic and functional microbial diversity with agriculture: How fragile is the Brazilian Cerrado?[J]. BMC Microbiology, 2016, 16: 42 http://europepmc.org/articles/PMC4794851/
[31]	WANG F, LIANG Y T, JIANG Y J, et al. Planting increases the abundance and structure complexity of soil core functional genes relevant to carbon and nitrogen cycling[J]. Scientific Reports, 2015, 5: 14345 http://www.nature.com/articles/srep14345
[32]	SUN Q Q, WANG R, HU Y X, et al. Spatial variations of soil respiration and temperature sensitivity along a steep slope of the semiarid Loess Plateau[J]. PLoS One, 2018, 13(4): e0195400 http://europepmc.org/abstract/MED/29624600
[33]	PROCTER A C, ELLIS J C, FAY P A, et al. Fungal community responses to past and future atmospheric CO₂ differ by soil type[J]. Applied and Environmental Microbiology, 2014, 80(23): 7364-7377 http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_PM25239904
[34]	SPATAFORA J W, CHANG Y, BENNY G L, et al. A phylum-level phylogenetic classification of zygomycete fungi based on genome-scale data[J]. Mycologia, 2016, 108(5): 1028-1046 http://www.ncbi.nlm.nih.gov/pubmed/27738200
[35]	GRYGANSKYI A P, HUMBER R A, SMITH M E, et al. Phylogenetic lineages in Entomophthoromycota[J]. Persoonia-Molecular Phylogeny and Evolution of Fungi, 2013, 30(1): 94-105 http://europepmc.org/articles/PMC3734969/