Volume 31 Issue 6
Jun.  2023
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CHEN X H, XU X Y, FU L Y, PAN Y J, FENG Y, CAI Z Q. Nitrogen acquirement strategy of different nitrogen forms in two pineapple cultivars[J]. Chinese Journal of Eco-Agriculture, 2023, 31(6): 895−903 doi: 10.12357/cjea.20220857
Citation: CHEN X H, XU X Y, FU L Y, PAN Y J, FENG Y, CAI Z Q. Nitrogen acquirement strategy of different nitrogen forms in two pineapple cultivars[J]. Chinese Journal of Eco-Agriculture, 2023, 31(6): 895−903 doi: 10.12357/cjea.20220857

Nitrogen acquirement strategy of different nitrogen forms in two pineapple cultivars

doi: 10.12357/cjea.20220857
Funds:  This study was supported by the National Natural Science Foundation of China (31971697), the Forestry Innovation Project in Guangdong (2021KJCX002), and the Rural Revitalization in Guangdong Province (2021-6844).
More Information
  • Corresponding author: E-mail: zhiquan.cai@126.com
  • Received Date: 2022-11-04
  • Accepted Date: 2023-01-12
  • Rev Recd Date: 2023-01-12
  • Available Online: 2023-02-07
  • Publish Date: 2023-06-10
  • Pineapple [Ananas comosus (Linn.) Merr.] is China’s third largest tropical fruit, with the largest planting area in Xuwen County, Guangdong Province. As one of the most important macronutrients, nitrogen is closely related to pineapple yield. However, the uptake preferences for different nitrogen forms in field-grown pineapple plants remain unclear. In this study, the morphological, physiological, and growth traits of plants with different ages were measured in two field-grown pineapple cultivars (‘Tainong 17’ and ‘Bali’) with different growth periods in April and September, respectively, in Xuwen County. In addition, nitrogen acquisition strategies for three different forms of nitrogen (ammonium nitrogen, nitrate nitrogen, and glycine) in the pineapple roots were determined using the stable isotope 15N tracer technique. The results indicated that the growth period of the ‘Tainong 17’ pineapple (16 months) was shorter than that of ‘Bali’ (20 months). During the fruit harvest period in April, compared with the ‘Bali’ pineapple (796 g fresh fruit weight per plant), ‘Tainong 17’ pineapple plants had lower yield (532 g fresh fruit weight per plant), root biomass, and P content; but had similar plant height, plant biomass per plant, leaf N and K contents, and specific leaf area. As an indicator of long-term water-use efficiency, the δ13C value ranging from −15.16‰ to −13.28‰, was higher in the leaves of ‘Tainong 17’ pineapple than that in ‘Bali’. Neither cultivar nor age greatly affected the leaf δ13C values. In April and September, there were significant differences in the different forms of nitrogen uptake between the two pineapple cultivars. The nitrogen uptake capacity of ‘Tainong 17’ pineapple was higher than that of ‘Bali’. The high acquirement capacity of nitrogen and water use efficiency of ‘Tainong 17’ pineapple is attributed to promoting photosynthesis and thus maintaining plant growth in a relatively short life cycle. Both pineapple cultivars preferred to acquire ammonium nitrogen (36.8%–64.6%), followed by glycine (23.2%–47.1%), and the uptake rate of nitrate nitrogen was the lowest (9.1%–31.5%). The nitrogen uptake rate of pineapple plants in the vegetative growth stage (5–8-month-old) was higher than that of plants in the fruit-harvesting stage. However, with increasing plant age, the contribution rate of ammonium nitrogen increased, whereas that of glycine gradually decreased. Across different pineapple cultivars and plant ages, the rates of different forms of nitrogen uptake were not linearly correlated with the soil nitrogen content or measured plant traits. To the best of our knowledge, this is the first study to show that the roots of field-grown pineapple plants can directly absorb organic nitrogen from the soil. Cultivar and plant growth stages of pineapples are important factors that affect nitrogen acquisition strategies. However, the linear relationships between the absorption rates of different forms of nitrogen and soil nitrogen content or measured plant traits were very weak. These results contribute to nitrogen fertilizer management in pineapple plantations.
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  • [1]
    王春晓, 凌飞, 鹿泽启, 等. 不同氮效率花生品种氮素累积与利用特征[J]. 中国生态农业学报(中英文), 2019, 27(11): 1706−1713

    WANG C X, LING F, LU Z Q, et al. Characteristics of nitrogen accumulation and utilization in peanuts (Arachis hypogaea) with different nitrogen use efficiencies[J]. Chinese Journal of Eco-Agriculture, 2019, 27(11): 1706−1713
    连盈, 卢娟, 胡成梅, 等. 低氮胁迫对谷子苗期性状的影响和耐低氮品种的筛选[J]. 中国生态农业学报(中英文), 2020, 28(4): 523−534

    LIAN Y, LU J, HU C M, et al. Effects of low nitrogen stress on foxtail millet seedling characteristics and screening of low nitrogen tolerant varieties[J]. Chinese Journal of Eco-Agriculture, 2020, 28(4): 523−534
    MA L N, XU X F, ZHANG C X, et al. Strong non-growing season N uptake by deciduous trees in a temperate forest: A 15N isotopic experiment[J]. Journal of Ecology, 2021, 109: 3752−3766 doi: 10.1111/1365-2745.13754
    CHAPIN F S Ⅲ, MOILANEN L, KIELLAND K. Preferential use of organic nitrogen for growth by a non-mycorrhizal arctic sedge[J]. Nature, 1993, 361(6408): 150−153 doi: 10.1038/361150a0
    NÄSHOLM T, HUSS-DANELL K, HÖGBERG P. Uptake of glycine by field grown wheat[J]. New Phytologist, 2001, 150(1): 59−63 doi: 10.1046/j.1469-8137.2001.00072.x
    ICHIHASHI Y, DATE Y, SHINO A, et al. Multi-omics analysis on an agroecosystem reveals the significant role of organic nitrogen to increase agricultural crop yield[J]. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117(25): 14552−14560 doi: 10.1073/pnas.1917259117
    FARZADFAR S, CONGREVES K A. Soil organic nitrogen: an overlooked but potentially significant contribution to crop nutrition[J]. Plant and Soil, 2021, 462(1): 7−23
    ZHANG J B, WANG J, MÜLLER C, et al. Ecological and practical significances of crop species preferential N uptake matching with soil N dynamics[J]. Soil Biology and Biochemistry, 2016, 103: 63−70 doi: 10.1016/j.soilbio.2016.08.009
    CUI J H, YU C Q, QIAO N, et al. Plant preference for NH4+ versus NO3 at different growth stages in an alpine agroecosystem[J]. Field Crops Research, 2017, 201: 192−199 doi: 10.1016/j.fcr.2016.11.009
    HENNERON L, KARDOL P, WARDLE D A, et al. Rhizosphere control of soil nitrogen cycling: a key component of plant economic strategies[J]. The New Phytologist, 2020, 228(4): 1269−1282 doi: 10.1111/nph.16760
    MERCIER H, KERBAUY G B, CARVALHO M T, et al. Growth and GDH and AAT isoenzyme patterns in terrestrial and epiphytic bromeliads as influenced by nitrogen source[J]. Selbyana, 1997, 18(1): 89−94
    ENDRES L, MERCIER H. Ammonium and urea as nitrogen sources for bromeliads[J]. Journal of Plant Physiology, 2001, 158(2): 205−212 doi: 10.1078/0176-1617-00170
    MAIA V M, PEGORARO R F, ASPIAZÚ I, et al. Diagnosis and management of nutrient constraints in pineapple[M]// SRIVASTAVA A K, HU C X. Fruit Crops: Diagnosis and Management of Nutrient Constraints. Amsterdam: Elsevier, 2020: 739–760
    PEGORARO R F, DE SOUZA B A M, MAIA V M, et al. Growth and production of irrigated Vitória pineapple grown in semi-arid conditions[J]. Revista Brasileira De Fruticultura, 2014, 36(3): 693−703 doi: 10.1590/0100-2945-265/13
    陈菁, 徐明岗, 孙光明, 等. 叶面追施硝态氮肥抑制菠萝营养生长效应[J]. 中国土壤与肥料, 2018(4): 82−86 doi: 10.11838/sfsc.20180413

    CHEN J, XU M G, SUN G M, et al. Effect of nitrate nitrogen on inhibiting the vegetative growth of pineapple[J]. Soil and Fertilizer Sciences in China, 2018(4): 82−86 doi: 10.11838/sfsc.20180413
    卢明, 剧虹伶, 洪珊, 等. 不同菠萝品种矿质养分的积累特性及利用效率[J]. 果树学报, 2017, 34(9): 1152−1160 doi: 10.13925/j.cnki.gsxb.20170072

    LU M, JU H L, HONG S, et al. A study on the mineral nutrient accumulation properties and use efficiency in different pineapple varieties[J]. Journal of Fruit Science, 2017, 34(9): 1152−1160 doi: 10.13925/j.cnki.gsxb.20170072
    张锡铜, 吴青松, 林文秋, 等. 18份菠萝种质果实外观性状比较分析[J]. 果树学报, 2022, 39(1): 78−85 doi: 10.13925/j.cnki.gsxb.20200569

    ZHANG X T, WU Q S, LIN W Q, et al. Comparative analysis of fruit appearance traits of 18 accessions in pineapple germplasm[J]. Journal of Fruit Science, 2022, 39(1): 78−85 doi: 10.13925/j.cnki.gsxb.20200569
    陈菁, 孙光明, 习金根, 等. 菠萝不同品种氮、磷、钾养分累积差异性研究[J]. 广东农业科学, 2010, 37(6): 87–88

    CHEN J, SUN G M, XI J G, et al. Study on the N, P, K accumulative rule of plantlets of different pinpeapple variety[J]. Guangdong Agricultural Sciences, 2010, 37(6): 87–88
    李常诚, 李倩茹, 徐兴良, 等. 不同林龄杉木氮素的获取策略[J]. 生态学报, 2016, 36(9): 2620−2625

    LI C C, LI Q R, XU X L, et al. Nitrogen acquisition strategies of Cunninghamia lanceolata at different ages[J]. Acta Ecologica Sinica, 2016, 36(9): 2620−2625
    ZHANG Z L, LI N, XIAO J, et al. Changes in plant nitrogen acquisition strategies during the restoration of spruce plantations on the eastern Tibetan Plateau, China[J]. Soil Biology and Biochemistry, 2018, 119: 50−58 doi: 10.1016/j.soilbio.2018.01.002
    董鸣. 陆地生物群落调查观测与分析[M]. 北京: 中国标准出版社, 1997

    DONG M. Survey, Observation and Analysis of Terristal Biocommunities[M]. Beijing: Standards Press of China, 1997
    LAMBERS H, OLIVEIRA R S. Plant Physiological Ecology[M]. Springer Cham: Springer, 2019
    MASCLAUX-DAUBRESSE C, DANIEL-VEDELE F, DECHORGNAT J, et al. Nitrogen uptake, assimilation and remobilization in plants: challenges for sustainable and productive agriculture[J]. Annals of Botany, 2010, 105(7): 1141−1157 doi: 10.1093/aob/mcq028
    FRESCHET G T, ROUMET C, COMAS L H, et al. Root traits as drivers of plant and ecosystem functioning: current understanding, pitfalls and future research needs[J]. The New Phytologist, 2021, 232(3): 1123−1158 doi: 10.1111/nph.17072
    MOREAU D, BARDGETT R D, FINLAY R D, et al. A plant perspective on nitrogen cycling in the rhizosphere[J]. Functional Ecology, 2019, 33(4): 540−552 doi: 10.1111/1365-2435.13303
    徐坤, 赵青春. 甜椒对不同形态氮素的吸收和分配[J]. 核农学报, 1999, 13(6): 339−342 doi: 10.3969/j.issn.1000-8551.1999.06.004

    XU K, ZHAO Q C. Absorption and distribution of different form of nitrogen in sweet pepper[J]. Journal of Nuclear Agricultural Sciences, 1999, 13(6): 339−342 doi: 10.3969/j.issn.1000-8551.1999.06.004
    LEROY C, CARRIAS J F, CÉRÉGHINO R, et al. The contribution of microorganisms and metazoans to mineral nutrition in bromeliads[J]. Journal of Plant Ecology, 2016, 9(3): 241−255 doi: 10.1093/jpe/rtv052
    MATIZ A, MIOTO P T, AIDAR M M, et al. Utilization of urea by leaves of bromeliad Vriesea gigantea under water deficit: much more than a nitrogen source[J]. Biologia Plantarum, 2017, 61(4): 751−762 doi: 10.1007/s10535-017-0721-z
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