Effects of chloroform fumigation on soil organic carbon mineralization in purple soil farmland

MA Han; WANG Xiaoguo

doi:10.12357/cjea.20220182

Volume 30 Issue 11

Nov. 2022

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Chinese Journal of Eco-Agriculture > 2022 > 30(11): 1819-1826. > DOI: 10.12357/cjea.20220182 CSTR: 32371.14.cjea.20220182

MA H, WANG X G. Effects of chloroform fumigation on soil organic carbon mineralization in purple soil farmland[J]. Chinese Journal of Eco-Agriculture, 2022, 30(11): 1819−1826. DOI: 10.12357/cjea.20220182

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Effects of chloroform fumigation on soil organic carbon mineralization in purple soil farmland

MA Han^{1, 2,},
WANG Xiaoguo^1, ,

1.
Key Laboratory of Mountain Surface Growth and Ecological Regulation, Chinese Academy of Sciences / Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Funds: The study was supported by the National Natural Science Foundation of China (42171067).

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Corresponding author:
WANG Xiaoguo, E-mail: xgwang@imde.ac.cn
Received Date: March 10, 2022
Accepted Date: May 09, 2022
Available Online: June 11, 2022

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Abstract

Abstract

In recent decades, mineralization of soil organic carbon has gradually become a focus due to greenhouse gas (GHG) emissions. The mineralization of soil organic carbon is mainly the decomposition of soil organic carbon under the action of microorganisms, which is an important pathway for soil organic carbon loss. Chloroform has strong sterilization power, and different microorganisms have different sensitivities to it. Furthermore, the soil microbial biomass and community composition can be changed by different fumigation durations. To explore the limiting factors of soil organic carbon mineralization in purple soil farmland, soils from plots with long-term application of pig manure treatment were selected for this laboratory incubation study. The effect of different soil microbial biomasses on soil organic carbon mineralization was investigated through varying the chloroform fumigation time and observing the changes in CO₂ emission rate under different treatments. The experiment included five treatments: fumigation for 24 h (C24), 2.5 h (C2.5), 1.5 h (C1.5), and 1 h (C1); and an unfumigated control (CK). The treatment not only changed the soil microbial biomass, but also greatly changed the soil microorganism community composition, which further verified the Regulation Gate hypothesis. The results showed that after fumigation, the soil microbial residues released microbial biomass carbon and the remaining microorganisms rapidly utilized this carbon source. Due to the availability of this new carbon source, the soil CO₂ emission rate increased rapidly. The variation trend in the soil CO₂ emission rate among different treatments was consistent. Due to the microbial residue carbon source, the CO₂ emission rate of fumigation treatment was higher than that of CK within 7 days of incubation, increasing rapidly to a maximum and then decreasing to a level comparable to the initial level. The order of the maximum values of the soil CO₂ emission rate among different treatments was C2.5>C24>C1.5>C1>CK. Compared with CK, the increases were 309.01%, 182.00%, 73.85%, and 30.45%, respectively. There were significant differences among the treatments (P<0.05). The soil CO₂ emission rate increased slowly and then decreased slowly during days 7–53 of the incubation. The average CO₂ emission rates of treatments C24, C2.5, C1.5, C1, and CK were 6.01±0.43, 5.94±0.29, 6.07±0.59, 5.78±0.49, and 6.23±0.13 μg∙g⁻¹∙h⁻¹, respectively. After 32 d, the rates of fumigation treatments were slightly lower than that of CK with no significant differences among different treatments. The variation in cumulative CO₂ emissions under different treatments conformed to the model y=at^b. The higher the maximum emission rate, the smaller the value of a and the larger the value of b. The b value of all treatments was less than 1, indicating that the cumulative emission increased with incubation time with a gradually slowing rate. The results of this study support the Regulatory Gate hypothesis of soil organic carbon mineralization, which states that the mineralization of soil organic carbon is unrelated to microbial biomass size, community composition, and activity in calcareous purple soil farmland treated with pig manure over a long period of time.
- Purple soil,
- Fumigation,
- Soil microorganism,
- Soil organic carbon mineralization

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[1]	SCHLESINGER W H. Evidence from chronosequence studies for a low carbon-storage potential of soils[J]. Nature, 1990, 348(6298): 232−234 doi: 10.1038/348232a0
[2]	方精云. 全球生态学: 气候变化与生态响应[M]. 北京: 高等教育出版社, 2000 FANG J Y. Global Ecology: Climate Change and Ecological Responses[M]. Beijing: Higher Education Press, 2000
[3]	李银坤, 陈敏鹏, 梅旭荣, 等. 土壤水分和氮添加对华北平原高产农田有机碳矿化的影响[J]. 生态学报, 2014, 34(14): 4037−4046 LI Y K, CHEN M P, MEI X R, et al. Effects of soil moisture and nitrogen addition on organic carbon mineralization in a high-yield cropland soil of the North China Plain[J]. Acta Ecologica Sinica, 2014, 34(14): 4037−4046
[4]	EKSCHMITT K, LIU M Q, VETTER S, et al. Strategies used by soil biota to overcome soil organic matter stability — why is dead organic matter left over in the soil?[J]. Geoderma, 2005, 128(1/2): 167−176
[5]	FONTAINE S, BAROT S. Size and functional diversity of microbe populations control plant persistence and long-term soil carbon accumulation[J]. Ecology Letters, 2005, 8(10): 1075−1087 doi: 10.1111/j.1461-0248.2005.00813.x
[6]	周莉, 李保国, 周广胜. 土壤有机碳的主导影响因子及其研究进展[J]. 地球科学进展, 2005, 20(1): 99−105 doi: 10.3321/j.issn:1001-8166.2005.01.016 ZHOU L, LI B G, ZHOU G S. Advances in controlling factors of soil organic carbon[J]. Advance in Earth Sciences, 2005, 20(1): 99−105 doi: 10.3321/j.issn:1001-8166.2005.01.016
[7]	BATJES N H. Options for increasing carbon sequestration in West African soils: an exploratory study with special focus on Senegal[J]. Land Degradation & Development, 2001, 12(2): 131−142
[8]	陈果, 刘岳燕, 姚槐应, 等. 一种测定淹水土壤中微生物生物量碳的方法: 液氯熏蒸浸提-水浴法[J]. 土壤学报, 2006, 43(6): 981−988 doi: 10.3321/j.issn:0564-3929.2006.06.015 CHEN G, LIU Y Y, YAO H Y, et al. A method for measuring microbial biomass C in waterlogged soil: chloroform fumigation extraction-water bath method[J]. Acta Pedologica Sinica, 2006, 43(6): 981−988 doi: 10.3321/j.issn:0564-3929.2006.06.015
[9]	BLANKINSHIP J C, BECERRA C A, SCHAEFFER S M, et al. Separating cellular metabolism from exoenzyme activity in soil organic matter decomposition[J]. Soil Biology and Biochemistry, 2014, 71: 68−75 doi: 10.1016/j.soilbio.2014.01.010
[10]	RENELLA G, LANDI L, NANNIPIERI P. Hydrolase activities during and after the chloroform fumigation of soil as affected by protease activity[J]. Soil Biology and Biochemistry, 2002, 34(1): 51−60 doi: 10.1016/S0038-0717(01)00152-3
[11]	DOMÍNGUEZ-MENDOZA C A, BELLO-LÓPEZ J M, NAVARRO-NOYA Y E, et al. Bacterial community structure in fumigated soil[J]. Soil Biology and Biochemistry, 2014, 73: 122−129 doi: 10.1016/j.soilbio.2014.02.012
[12]	JENKINSON D S, POWLSON D S. The effects of biocidal treatments on metabolism in soil —Ⅰ. Fumigation with chloroform[J]. Soil Biology and Biochemistry, 1976, 8(3): 167−177 doi: 10.1016/0038-0717(76)90001-8
[13]	KEMMITT S J, LANYON C V, WAITE I S, et al. Mineralization of native soil organic matter is not regulated by the size, activity or composition of the soil microbial biomass — A new perspective[J]. Soil Biology and Biochemistry, 2008, 40(1): 61−73 doi: 10.1016/j.soilbio.2007.06.021
[14]	VAN ELSAS J D, HILL P, CHRONÁKOVÁ A, et al. Survival of genetically marked Escherichia coli O157: H7 in soil as affected by soil microbial community shifts[J]. The ISME Journal, 2007, 1(3): 204−214 doi: 10.1038/ismej.2007.21
[15]	JOERGENSEN R G, BROOKES P C, JENKINSON D S. Survival of the soil microbial biomass at elevated temperatures[J]. Soil Biology and Biochemistry, 1990, 22(8): 1129−1136 doi: 10.1016/0038-0717(90)90039-3
[16]	ZHOU X Y, CHEN L, XU J M, et al. Soil biochemical properties and bacteria community in a repeatedly fumigated-incubated soil[J]. Biology and Fertility of Soils, 2020, 56(5): 619−631 doi: 10.1007/s00374-020-01437-0
[17]	ZHOU X Y, CHEN L, LI Y, et al. Abiotic processes dominate soil organic matter mineralization: investigating the regulatory gate hypothesis by inoculating a previously fumigated soil with increasing fresh soil inocula[J]. Geoderma, 2020, 373: 114400 doi: 10.1016/j.geoderma.2020.114400
[18]	中国科学院成都分院土壤研究室. 中国紫色土上篇[M]. 北京: 科学出版社, 1991: 340 Laboratory of Soil, Chengdu Branch, Chinese Academy of Sciences. Purple Soil in China (A) [M]. Beijing: Science Press, 1991: 340
[19]	鲁如坤. 土壤农业化学分析方法[M]. 北京: 中国农业科技出版社, 2000: 228–232 LU R K. Soil and Agricultural Chemistry Analysis[M]. China Agriculture Scientech Press, 2000: 228–232
[20]	孔维栋, 朱永官, 傅伯杰, 等. 农业土壤微生物基因与群落多样性研究进展[J]. 生态学报, 2004, 24(12): 2894−2900 doi: 10.3321/j.issn:1000-0933.2004.12.034 KONG W D, ZHU Y G, FU B J, et al. A review on microbial gene and community diversity in agricultural soil[J]. Acta Ecologica Sinica, 2004, 24(12): 2894−2900 doi: 10.3321/j.issn:1000-0933.2004.12.034
[21]	中华人民共和国农业农村部. GB 39228—2020 土壤微生物生物量的测定熏蒸浸提法[S]. 北京: 中国标准出版社, 2020 Ministry of Agriculture and Rural Affairs of the People’s Republic of China. GB 39228—2020 Determination of Soil Microbial Biomass—Fumigation-Extraction Method[S]. Beijing: China Standards Press, 2020
[22]	刘雨晴, 朱小琴, 胡会峰, 等. 熏蒸浸提法测定碱性土微生物生物量碳初探[J]. 土壤, 2018, 50(3): 640−644 LIU Y Q, ZHU X Q, HU H F, et al. Preliminary study of fumigation extraction in microbial biomass carbon of alkali soil[J]. Soils, 2018, 50(3): 640−644
[23]	DUNGAIT J A J, HOPKINS D W, GREGORY A S, et al. Soil organic matter turnover is governed by accessibility not recalcitrance[J]. Global Change Biology, 2012, 18(6): 1781−1796 doi: 10.1111/j.1365-2486.2012.02665.x
[24]	BROOKES P C, CHEN Y F, CHEN L, et al. Is the rate of mineralization of soil organic carbon under microbiological control?[J]. Soil Biology and Biochemistry, 2017, 112: 127−139 doi: 10.1016/j.soilbio.2017.05.003
[25]	KUZYAKOV Y, BLAGODATSKAYA E, BLAGODATSKY S. Comments on the paper by Kemmitt et al. (2008) ‘Mineralization of native soil organic matter is not regulated by the size, activity or composition of the soil microbial biomass — A new perspective’ [Soil Biology & Biochemistry 40, 61–73]: The biology of the Regulatory Gate[J]. Soil Biology and Biochemistry, 2009, 41(2): 435−439 doi: 10.1016/j.soilbio.2008.07.023
[26]	LI Y H, SHAHBAZ M, ZHU Z K, et al. Oxygen availability determines key regulators in soil organic carbon mineralisation in paddy soils[J]. Soil Biology and Biochemistry, 2021, 153: 108106 doi: 10.1016/j.soilbio.2020.108106
[27]	PANETTIERI M, GUIGUE J, CHEMIDLIN PREVOST-BOURÉ N, et al. Grassland-cropland rotation cycles in crop-livestock farming systems regulate priming effect potential in soils through modulation of microbial communities, composition of soil organic matter and abiotic soil properties[J]. Agriculture, Ecosystems & Environment, 2020, 299: 106973
[28]	FONTAINE S, BAROT S, BARRÉ P, et al. Stability of organic carbon in deep soil layers controlled by fresh carbon supply[J]. Nature, 2007, 450(7167): 277−280 doi: 10.1038/nature06275
[29]	WARDLE D A. A comparative assessment of factors which influence microbial biomass carbon and nitrogen levels in soil[J]. Biological Reviews, 1992, 67(3): 321−358 doi: 10.1111/j.1469-185X.1992.tb00728.x
[30]	LIANG C, SCHIMEL J P, JASTROW J D. The importance of anabolism in microbial control over soil carbon storage[J]. Nature Microbiology, 2017, 2: 17105 doi: 10.1038/nmicrobiol.2017.105
[31]	SCHWEIGERT M, HERRMANN S, MILTNER A, et al. Fate of ectomycorrhizal fungal biomass in a soil bioreactor system and its contribution to soil organic matter formation[J]. Soil Biology and Biochemistry, 2015, 88: 120−127 doi: 10.1016/j.soilbio.2015.05.012
[32]	KINDLER R, MILTNER A, RICHNOW H H, et al. Fate of gram-negative bacterial biomass in soil—Mineralization and contribution to SOM[J]. Soil Biology and Biochemistry, 2006, 38(9): 2860−2870 doi: 10.1016/j.soilbio.2006.04.047
[33]	LIANG C, AMELUNG W, LEHMANN J, et al. Quantitative assessment of microbial necromass contribution to soil organic matter[J]. Global Change Biology, 2019, 25(11): 3578−3590 doi: 10.1111/gcb.14781
[34]	ROUSK J, BÅÅTH E. Fungal biomass production and turnover in soil estimated using the acetate-in-ergosterol technique[J]. Soil Biology and Biochemistry, 2007, 39(8): 2173−2177 doi: 10.1016/j.soilbio.2007.03.023

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Effects of chloroform fumigation on soil organic carbon mineralization in purple soil farmland

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