Abstract: Effects of four cultural methods on growth and differentiation of plantlets and microtuber induction were studied under different states，volumes and adding time of culture media．The results showed that the liquid media which were used two times and of which volume was reduced were much better than the,solid media．The significance of difference among various growth indexes of plantlets was notable extremely．They could be seen mainly from these aspects：the stem diameter increased 0.89cm ，the number of available node increased 2，the fresh biomass and dry mass were improved by 4．15g and 0．27g，respectively，meantime，the period of formation of the available plantlets was reduced by 5．5d．The microtuberization time could be advanced by 2～3 d，the No．of tuber per shoot was 2 and the weight per tuber was 100～1000mg．More importantly．the medium cost reduced by 72.42％．