杀虫剂胁迫对白菜生理生化物质的影响

Effect of insecticide stress on physiological and biochemical indicators of Brassica campestris L.

  • 摘要: 试验研究了4种杀虫剂(BT、阿维菌素、锐劲特、高效氯氰菊酯)对白菜5个主要生理生化指标(叶绿素含量、可溶性糖含量、游离氨基酸含量、SOD活性、POD活性)的影响,结果表明:施药后14 d内,各杀虫剂处理白菜叶片叶绿素含量与对照相比均有不同程度下降,施药后21 d各处理对叶绿素含量的影响有显著性差异,其顺序为锐劲特处理>高效氯氰菊酯处理>阿维菌素处理>BT处理>对照。锐劲特及高效氯氰菊酯处理7 d后,白菜叶片可溶性糖含量较高,而阿维菌素处理21 d后,叶片中可溶性糖含量显著高于其他处理和对照。药剂处理后21 d,白菜叶片苏氨酸、异亮氨酸、苯丙氨酸含量明显高于对照,而缬氨酸、组氨酸含量明显低于对照。施杀虫剂对白菜叶片中氨基酸的影响在药后14 d较为强烈,锐劲特处理影响程度较小,高效氯氰菊酯处理影响较大。施药后7 d,阿维菌素处理和BT处理白菜叶片SOD活性显著高于其他处理和对照,施药后14 d以阿维菌素处理最高,施药后21 d各处理均较对照低。施药后白菜叶片POD活性总体呈上升趋势,其中以锐劲特处理和阿维菌素处理增幅较大,分别达598%和247%。研究结果为合理选择药剂防治白菜害虫提供理论和实践依据。

     

    Abstract: The effect of four insecticides (Bacillus thuringiensis, Abamectin, Fipronil, and Beta-cypwrmethrin) on five principal physiological and biochemical indicators (chlorophyll, soluble sugar and free amino acid content, and SOD and POD activity) of B.campestris was researched. The results show that chlorophyll content declines compared with the control treatment 14 days after insecticide application. After three weeks of insecticide application, each treatment shows a significant difference in chlorophyll content in the order as follows: Fipronil treatment > Beta-cypwrmethrin treatment > Abamectin treatment > Bacillus thuringiensis treatment > control treatment. Soluble sugar content is relatively high after 7 days in Fipronil and Beta-cypwrmethrin respectively,while in Abamectin, is significant higher than that in other treatments 21 days after insecticide application. In comparison with the control treatment, THR, ILE and PHE contents are markedly high, while VAL and HIS contents are markedly low three weeks after insecticide application. On the whole, the effect of insecticide stress on free amino acids in the leaves of B.campestris relatively intensifies after 14 days with light Fipronil stress effect, but serious Beta-cypwrmethrin stress effect. Seven days after insecticide application, SOD activity in Abamectin treatment and BT treatment significant increases over that in other treatments. SOD activity is simply highest in Abmectin treatment after two weeks, while all treatments have less SOD activity than the control three weeks after application. POD activity rises on the average, and the range of increase in Fipronil treatment and Abamectin treatment is a little high, reaching 598% and 247% respectively. This study provides an important theoretical and practical basis for reasonable selection of insecticides to control B.campestris pests.

     

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