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铅胁迫下接种AM真菌对龙葵根际土壤微生物群落代谢特征的影响

韩娟 张向前 赵金莉

韩娟, 张向前, 赵金莉. 铅胁迫下接种AM真菌对龙葵根际土壤微生物群落代谢特征的影响[J]. 中国生态农业学报(中英文), 2019, 27(4): 545-553. doi: 10.13930/j.cnki.cjea.181036
引用本文: 韩娟, 张向前, 赵金莉. 铅胁迫下接种AM真菌对龙葵根际土壤微生物群落代谢特征的影响[J]. 中国生态农业学报(中英文), 2019, 27(4): 545-553. doi: 10.13930/j.cnki.cjea.181036
HAN Juan, ZHANG Xiangqian, ZHAO Jinli. Effect of arbuscular mycorrhizal fungi on metabolic characteristics of microbial community in Solanum nigrum rhizosphere soil with lead stress[J]. Chinese Journal of Eco-Agriculture, 2019, 27(4): 545-553. doi: 10.13930/j.cnki.cjea.181036
Citation: HAN Juan, ZHANG Xiangqian, ZHAO Jinli. Effect of arbuscular mycorrhizal fungi on metabolic characteristics of microbial community in Solanum nigrum rhizosphere soil with lead stress[J]. Chinese Journal of Eco-Agriculture, 2019, 27(4): 545-553. doi: 10.13930/j.cnki.cjea.181036

铅胁迫下接种AM真菌对龙葵根际土壤微生物群落代谢特征的影响

doi: 10.13930/j.cnki.cjea.181036
基金项目: 

河北省自然科学基金项目 C2017201044

河北大学大学生创新训练项目 2017126

详细信息
    作者简介:

    韩娟, 主要从事菌根生物技术方面的研究。E-mail:junhan_hbu@126.com

    通讯作者:

    赵金莉, 主要从事土壤生态学方面的研究。E-mail:plant@hbu.edu.cn

  • 中图分类号: S154.3

Effect of arbuscular mycorrhizal fungi on metabolic characteristics of microbial community in Solanum nigrum rhizosphere soil with lead stress

Funds: 

the Natural Science Foundation of Hebei Province C2017201044

the Student Innovation Training Project of Hebei University 2017126

More Information
  • 摘要: 为从微生态角度探索接种AM真菌对铅污染根际土壤微生物群落代谢特征的影响,以摩西管柄囊霉(Funneliformis mosseae)和幼套近明囊霉(Claroideoglomus etunicatum)为接种菌剂,在温室盆栽条件下以Pb4+含量为0 mg·kg-1、200 mg·kg-1、400 mg·kg-1和800 mg·kg-1土壤种植龙葵(Solanum nigrum)10周后采集根际土壤,采用Biolog微平板法测定龙葵根际土壤微生物群落的代谢变化。结果表明:1)微生物平均代谢活性(AWCD)随铅浓度增加呈降—升—降趋势;接种AM真菌显著提高了铅胁迫下根际土壤微生物的AWCD值,仅中浓度(400 mg·kg-1)下未达显著差异。2)中浓度铅处理能显著提高根际土壤微生物对糖类及其衍生物、氨基酸类、脂肪酸和脂类及代谢产物类四大类碳源底物利用能力;接种AM真菌,高浓度(800 mg·kg-1)铅处理显著提高了根际土壤微生物对氨基酸类底物的利用能力。3)铅胁迫下接种AM真菌提高了根际土壤微生物多样性指数,在中浓度下丰富度指数、Shannon-Wiener多样性指数、Simpson优势度指数均达显著水平。4)主成分分析显示,代谢产物类在PC1和PC2中种类最多,分别为6种和4种;糖类及其衍生物在PC3中种类最多(5种)。5)在铅胁迫和接种AM真菌共同作用下,微生物碳源利用主要受铅浓度调节,并且二者对微生物碳源利用具有显著交互效应。综上可知,接种AM真菌能够提高铅胁迫下龙葵根际土壤微生物多样性指数,增强根际土壤微生物对碳源底物的利用能力。该研究为进一步探究AM真菌强化植物联合修复技术提供了依据。
  • 图  1  菌剂接种及播种方式

    Figure  1.  Method of inoculation and seeding

    图  2  接种AM真菌对铅胁迫下龙葵根际土壤微生物平均颜色变化率(AWCD)的影响

    CK:未接种AM真菌, Pb4+为0 mg·kg-1; AM:接种AM真菌; Pb1: Pb4+浓度为200 mg·kg-1(低浓度); Pb2: Pb4+浓度为400 mg·kg-1(中浓度); Pb3: Pb4+浓度为800 mg·kg-1(高浓度)。*和**分别表示在P < 0.05和P < 0.01水平差异显著。

    Figure  2.  Effect of AM fungi inoculation on the average well color development (AWCD) of microbia in rhizosphere soil of Solanum nigrum under lead stress

    CK: no AM with 0 mg·kg-1 Pb4+; AM: inoculation of AM; Pb1: low Pb4+ concentration (200 mg·kg-1); Pb2: medium Pb4+ concentration (400 mg·kg-1); Pb3: high Pb4+ concentration (800 mg·kg-1). * and ** mean significant difference at P < 0.05 and P < 0.01 levels, respectively.

    图  3  接种AM真菌和铅胁迫下龙葵根际土壤微生物群落碳源代谢特征的主成分分析

    CK:未接种AM真菌, Pb4+为0 mg·kg-1; AM:接种AM真菌; Pb1: Pb4+浓度为200 mg·kg-1(低浓度); Pb2: Pb4+浓度为400 mg·kg-1(中浓度); Pb3: Pb4+浓度为800 mg·kg-1(高浓度)。

    Figure  3.  Principal component analysis for carbon source utilization of soil microbes in Solanum nigrum rhizosphere under inoculation of AM and lead stress

    CK: no AM with 0 mg·kg-1 Pb4+; AM: inoculation AM; Pb1: low Pb4+ concentration (200 mg·kg-1); Pb2: medium Pb4+ concentration (400 mg·kg-1); Pb3: high Pb4+ concentration (800 mg·kg-1).

    表  1  接种AM真菌和铅胁迫对各测定指标影响的方差分析

    Table  1.   ANOVA analysis results of effects of AM inoculation and lead stress on the investigated indexes of the experiment

    指标
    Index
    变异来源Source of variation
    AM接种(AM)
    AM inoculation
    铅处理(Pb)
    Pb treatment
    AMxPb
    F P F P F P
    平均颜色变化率Average well color development (AWCD) 11.456 ** 5.851 ** 3.793 *
    物种多样性指数Shannon-Wiener diversity index (H') 24.750 *** 1.031 NS 0.269 NS
    优势度指数Simpson dominance index (D) 30.727 *** 0.438 NS 0.511 NS
    丰富度指数Species richness index (S) 19.678 *** 2.444 NS 0.611 NS
    McIntosh均匀度指数McIntosh evenness index (U) 5.270 * 5.232 ** 3.428 *
    糖类及其衍生物Carbohydrate and its derivates 0.030 NS 22.922 *** 23.623 ***
    氨基酸类Amino acid 0.268 NS 4.380 * 3.951 *
    脂肪酸及脂类Fatty acid and lipid 0.072 NS 7.814 ** 10.344 ***
    代谢产物Metabolites 5.709 * 6.952 ** 6.538 **
    NS:不显著; *:显著水平为P < 0.05; **:显著水平为P < 0.01; ***:显著水平为P < 0.001。NS: no significant difference; *: significant difference at P < 0.05; **: significant difference at P < 0.01; ***: significant difference at P < 0.001.
    下载: 导出CSV

    表  2  接种AM真菌对铅胁迫下龙葵根际土壤微生物功能多样性指数的影响

    Table  2.   Effect of AM fungi inoculation on soil microbial function diversity indexes of Solanum nigrum rhizosphere soil under lead stress

    处理
    Treatment
    丰富度指数
    Species richness index
    Shannon-Wiener多样性指数
    Shannon-Wiener diversity index
    Simpson优势度指数
    Simpson dominance index
    McIntosh均匀度指数
    McIntosh evenness index
    CK 24.67±1.33aA 3.175 4±0.002 9aA 0.951 5±0.000 4aA 3.760 4±0.045 0aA
    Pb1 22.67±0.88bA 3.079 0±0.054 5aA 0.945 6±0.003 9aA 3.494 7±0.320 1aA
    Pb2 24.67±2.03aA 3.128 1±0.085 8aA 0.950 0±0.004 7aA 3.037 3±0.654 4bA
    Pb3 24.00±1.15aA 3.139 0±0.022 3aA 0.948 9±0.001 2aA 3.593 7±0.337 7aA
    AM+CK 29.67±0.33aB 3.303 7±0.024 3aA 0.960 0±0.001 4aB 5.774 5±0.154 0aB
    AM+Pb1 26.00±1.15bA 3.263 7±0.008 5aB 0.957 4±0.000 7aB 4.225 0±0.248 8bA
    AM+Pb2 29.00±0.58aB 3.277 4±0.027 1aB 0.957 9±0.001 6aB 4.771 1±0.345 4abA
    AM+Pb3 26.33±0.33bA 3.238 4±0.009 1aA 0.955 8±0.000 5aA 4.212 7±0.337 4bA
    CK:未接种AM真菌, Pb4+为0 mg·kg-1; AM:接种AM真菌; Pb1: Pb4+浓度为200 mg·kg-1(低浓度); Pb2: Pb4+浓度为400 mg·kg-1(中浓度); Pb3: Pb4+浓度为800 mg·kg-1(高浓度)。同列不同小写字母分别表示接种AM真菌或未接种AM真菌处理下不同Pb4+浓度间差异显著(P < 0.05);同列不同大写字母表示同一Pb4+浓度下接种AM和不接种AM处理间差异显著(P < 0.05)。CK: no AM with 0 mg·kg-1 Pb4+; AM: inoculation of AM; Pb1: low Pb4+ concentration (200 mg·kg-1); Pb2: medium Pb4+ concentration (400 mg·kg-1); Pb3: high Pb4+ concentration (800 mg·kg-1). Different lowercase letters in the same column indicate significant differences among different concentrations of Pb4+ at P < 0.05. Different capital letters in the same column indicate significant differences between AM inoculation and no inoculation under the same Pb4+ concentration at P < 0.05.
    下载: 导出CSV

    表  3  接种AM真菌对铅胁迫下龙葵根际土壤微生物碳源利用强度的影响

    Table  3.   Effect of AM fungi inoculation on carbon source utilization of soil microbes in Solanum nigrum rhizosphere under lead stress

    处理
    Treatment
    碳源类型Carbon sources
    糖类及其衍生物
    Carbohydrate and derivatives
    氨基酸类
    Amino acids
    脂肪酸和脂类
    Fatty acid and lipids
    代谢产物
    Metabolites
    CK 0.664 4±0.046 9aA 0.573 5±0.028 6aA 0.575 9±0.062 6aA 0.315 5±0.022 9aA
    Pb1 0.553 4±0.012 5aA 0.722 7±0.048 2aA 0.507 2±0.071 7aA 0.334 8±0.080 7aA
    Pb2 0.914 3±0.062 8bA 0.808 8±0.063 1aA 0.604 0±0.131 2aA 0.503 2±0.105 9bA
    Pb3 0.614 3±0.097 0aA 0.554 0±0.054 5aA 0.549 7±0.076 1aA 0.305 7±0.032 6aA
    AM+CK 0.981 6±0.041 1aB 1.032 7±0.015 4aB 0.941 2±0.061 8aB 0.774 4±0.018 6aB
    AM+Pb1 0.748 5±0.057 0abA 0.744 3±0.036 9bA 0.640 9±0.042 1bA 0.479 0±0.060 4bA
    AM+Pb2 0.842 3±0.066 1abA 0.848 2±0.050 9abA 0.687 9±0.024 8bA 0.571 7±0.039 4bA
    AM+Pb3 0.683 4±0.032 4bA 0.849 2±0.119 7abB 0.637 5±0.0577 bA 0.444 9±0.070 4bA
    CK:未接种AM真菌, Pb4+为0 mg·kg-1; AM:接种AM真菌; Pb1: Pb4+浓度为200 mg·kg-1(低浓度); Pb2: Pb4+浓度为400 mg·kg-1(中浓度); Pb3: Pb4+浓度为800 mg·kg-1(高浓度)。同列不同小写字母分别表示接种AM真菌或未接种AM真菌处理下不同Pb4+浓度间差异显著(P < 0.05);同列不同大写字母表示同一Pb4+浓度下接种AM和不接种AM处理间差异显著(P < 0.05)。CK: no AM with 0 mg·kg-1 Pb4+; AM: inoculation of AM; Pb1: low Pb4+ concentration (200 mg·kg-1); Pb2: medium Pb4+ concentration (400 mg·kg-1); Pb3: high Pb4+ concentration (800 mg·kg-1). Different lowercase letters in the same column indicate significant differences among concentrations of Pb4+ at P < 0.05. Different capital letters in the same column indicate significant differences between AM inoculation and no inoculation under the same Pb4+ concentration at P < 0.05.
    下载: 导出CSV

    表  4  接种AM真菌和铅胁迫下龙葵根际土壤微生物Biolog-ECO板上碳源利用的主成分载荷矩阵

    Table  4.   Principal components loading matrix for soil microbes carbon source utilization of Biolog-ECO in Solanum nigrum rhizosphere under inoculation of AM and lead stress

    碳源编号
    Code of carbon source
    碳源类别
    Carbon sources
    底物
    Substrate
    提取的主成分
    Principal component of extraction
    PC1 PC2 PC3 PC4 PC5 PC6
    A2 糖类及其衍生物
    Saccharides and derivates
    -甲基D-葡萄糖β-methyl-D-glucoside -0.112 0.167 0.072 -0.968 0.021 -0.112
    A3 D-半乳糖酸γ内酯D-galactonic acid γ-lactone 0.137 0.286 0.624 -0.060 0.254 0.653
    B2 D-木糖D-xylose 0.717 0.452 0.369 -0.186 0.316 0.014
    B3 D-半乳糖醛酸D-galacturonic acid -0.492 0.349 0.292 0.159 -0.113 0.709
    C2 i-赤藓糖醇i-erythritol 0.577 0.357 0.225 0.101 0.614 -0.316
    D2 D-甘露醇D-mannitol 0.575 0.303 0.751 -0.011 -0.040 0.104
    E1 α环状糊精α-cyclodextrin 0.768 0.353 0.206 0.226 0.087 -0.014
    E2 N-乙酰基-D-葡萄糖氨N-acetyl-D-glucosamine 0.096 -0.123 0.889 0.168 0.202 0.028
    F1 肝糖Glycogen 0.159 0.719 0.559 -0.080 0.276 0.250
    F2 D-氨基葡萄糖酸D-glucosaminic acid 0.588 0.277 0.178 0.723 0.131 -0.078
    G1 D-纤维二糖D-cellobiose 0.400 0.216 0.845 0.120 0.231 0.107
    H1 α-D-乳糖α-D-lactose 0.407 0.177 0.309 0.079 0.804 0.218
    A4 氨基酸类
    Amino acid
    L-精氨酸L-arginine 0.377 0.503 0.200 0.741 0.089 -0.090
    B4 L-天冬酰胺L-asparagine 0.206 -0.414 -0.815 0.249 0.178 0.077
    C4 L-苯丙氨酸L-phenylalanine -0.045 0.397 0.774 -0.323 -0.346 0.129
    D4 L-丝氨酸L-serine 0.095 0.061 0.833 0.429 0.059 0.309
    E4 L-苏氨酸L-threonine 0.795 0.525 0.139 0.080 0.137 -0.098
    F4 甘氨酰-L-谷氨酸Glycyl-L-glutamic acid 0.380 0.761 0.109 0.402 0.303 0.099
    B1 脂肪酸和脂类
    Fatty acids and lipids
    丙酮酸甲酯Pyruvic acid methyl ester 0.795 0.258 0.035 0.488 0.150 0.198
    C1 吐温40 Tween40 0.367 0.809 0.095 -0.257 0.206 0.299
    D1 吐温80 Tween 80 0.325 0.835 0.386 0.131 0.034 0.170
    E3 γ-羟基丁酸γ-hydroxybutyric acid 0.737 0.316 -0.095 0.492 0.216 -0.156
    F3 衣康酸Itaconic acid 0.193 0.863 0.405 0.004 -0.076 -0.207
    C3 代谢产物类
    Metabolites
    2-羟基苯甲酸2-hydroxy benzoic acid 0.968 -0.100 0.015 0.107 0.161 0.019
    D3 4-羟基苯甲酸4-hydroxy benzoic acid 0.554 0.671 0.310 0.377 -0.017 0.024
    G2 葡萄糖-1-磷酸盐Glucose-1-phosphate 0.817 0.565 0.031 0.047 -0.072 -0.062
    G3 α-丁酮酸α-ketobutyric acid 0.768 0.227 0.134 0.318 0.472 -0.127
    G4 苯乙胺Phenylethylamine 0.071 0.302 0.879 0.066 0.345 -0.008
    H2 D, L-a-磷酸甘油D, L-α-glycerol phosphate 0.163 0.947 0.016 0.126 0.215 0.146
    H3 D-苹果酸D-malic acid 0.577 0.672 0.317 0.184 -0.194 0.182
    H4 腐胺Putrescine 0.641 0.209 0.380 0.428 0.248 0.396
    下载: 导出CSV
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出版历程
  • 收稿日期:  2018-11-29
  • 录用日期:  2018-12-05
  • 刊出日期:  2019-04-01

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