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硒砂瓜连作对土壤真菌群落结构的影响

冯翠娥 岳思君 简阿妮 陈丽萍 郭洋 郑蕊 苏建宇

冯翠娥, 岳思君, 简阿妮, 陈丽萍, 郭洋, 郑蕊, 苏建宇. 硒砂瓜连作对土壤真菌群落结构的影响[J]. 中国生态农业学报(中英文), 2019, 27(4): 537-544. doi: 10.13930/j.cnki.cjea.180746
引用本文: 冯翠娥, 岳思君, 简阿妮, 陈丽萍, 郭洋, 郑蕊, 苏建宇. 硒砂瓜连作对土壤真菌群落结构的影响[J]. 中国生态农业学报(中英文), 2019, 27(4): 537-544. doi: 10.13930/j.cnki.cjea.180746
FENG Cui'e, YUE Sijun, JIAN Ani, CHEN Liping, GUO Yang, ZHENG Rui, SU Jianyu. The effect of continuous cropping of selenium melon on soil fungal community structure[J]. Chinese Journal of Eco-Agriculture, 2019, 27(4): 537-544. doi: 10.13930/j.cnki.cjea.180746
Citation: FENG Cui'e, YUE Sijun, JIAN Ani, CHEN Liping, GUO Yang, ZHENG Rui, SU Jianyu. The effect of continuous cropping of selenium melon on soil fungal community structure[J]. Chinese Journal of Eco-Agriculture, 2019, 27(4): 537-544. doi: 10.13930/j.cnki.cjea.180746

硒砂瓜连作对土壤真菌群落结构的影响

doi: 10.13930/j.cnki.cjea.180746
基金项目: 

国家自然科学基金项目 31360025

国家自然科学基金项目 31560418

宁夏回族自治区重点研发计划项目 2017BY081

详细信息
    作者简介:

    冯翠娥, 主要研究方向为微生物技术与工程。E-mail:13519291697@163.com

    通讯作者:

    岳思君, 主要研究方向为微生物学, E-mail:sijunyue@126.com

    苏建宇, 主要研究方向为工业微生物, E-mail:su_jy@nxu.edu.cn

  • 中图分类号: S154.3

The effect of continuous cropping of selenium melon on soil fungal community structure

Funds: 

the National Natural Science Foundation of China 31360025

the National Natural Science Foundation of China 31560418

Ningxia Key Research and Development Program 2017BY081

More Information
  • 摘要: 硒砂瓜是宁夏地区重要的经济作物,其连作严重影响硒砂瓜产量和品质。目前硒砂瓜连作对土壤真菌群落结构的影响尚不清楚。本研究采用Illumina MiSeq高通量测序技术,探讨硒砂瓜连作对土壤真菌群落结构的影响。研究发现,硒砂瓜连作土壤中真菌群落多样性指数和丰富度指数随连作年限的增加先上升后下降。供试土壤样本中共检测到真菌8门、25纲、244属,其中子囊菌门(Ascomycota)、接合菌门(Zygomycota)是优势菌门,占90%以上。与对照相比,连作30年土壤中子囊菌门丰度下降32.51%,接合菌门丰度上升29.89%。供试土壤中真菌主要的优势属为被孢霉属(Mortierella)、绿僵菌属(Metarhiziun)、假霉样真菌属(Pseudallescheria)、镰刀菌属(Fusarium)和青霉属(Penicillium)。与对照相比,连作5年土壤中假霉样真菌属丰度增加45.81%,连作10年土壤中镰刀菌属丰度增加26.74%,连作15年土壤中绿僵菌属下降26.83%,连作20年土壤中青霉属增加29.68%,连作25年土壤中绿僵菌属减少18.30%,连作30年土壤中被孢霉属丰度上升29.89%。土壤理化性质与硒砂瓜连作年限间无显著相关性,而与土壤真菌群落结构存在显著的相关性。土壤全磷、碱解氮、有效磷含量是影响土壤真菌群落最主要的因素。研究结果表明,导致硒砂瓜连作障碍的主要原因不是土壤理化性质变化,而是土壤真菌群落结构的改变。研究结果可为硒砂瓜土传病害的生物防治提供参考依据。
  • 图  1  硒砂瓜连作土壤样本在OUT水平Sobs指数稀释曲线

    T0、T5、T10、T15、T20、T25、T30分别指未种植硒砂瓜处理及连作5年、10年、15年、20年、25年和30年处理。

    Figure  1.  Rarefaction curves of OUT level Sobs index in soil samples of selenium-melon continuous cropping

    T0, T5, T10, T15, T20, T25, and T30 refer to the treatments of non-planting and continuous cropping for 5, 10, 15, 20, 25 and 30 years of selenium melon, respectively.

    图  2  硒砂瓜不同连作年限土壤真菌群落在门水平的相对丰度

    T0、T5、T10、T15、T20、T25、T30分别指未种植硒砂瓜处理及连作5年、10年、15年、20年、25年和30年处理。

    Figure  2.  Relative abundances of soil fungal communities at phylum level in different continuous cropping years of selenium-melon

    T0, T5, T10, T15, T20, T25, and T30 refer to the treatments of non-planting and continuous cropping for 5, 10, 15, 20, 25 and 30 years of selenium melon, respectively.

    图  3  硒砂瓜不同连作年限土壤真菌群落在纲水平相对丰度

    T0、T5、T10、T15、T20、T25、T30分别指未种植硒砂瓜处理及连作5年、10年、15年、20年、25年和30年处理。

    Figure  3.  Relative abundances of soil fungal communities at class level in different continuous cropping years of selenium-melon

    T0, T5, T10, T15, T20, T25, and T30 refer to the treatments of non-planting and continuous cropping for 5, 10, 15, 20, 25 and 30 years of selenium melon, respectively.

    图  4  硒砂瓜不同连作年限土壤真菌群落在属水平Heatmap图

    T0、T5、T10、T15、T20、T25、T30分别指未种植硒砂瓜处理及连作5年、10年、15年、20年、25年和30年处理。

    Figure  4.  Heatmap of soil fungal community at genus level in different continuous cropping years of selenium-melon

    T0, T5, T10, T15, T20, T25, and T30 refer to the treatments of non-planting and continuous cropping for 5, 10, 15, 20, 25 and 30 years of selenium melon, respectively.

    图  5  硒砂瓜不同连作年限土壤真菌基于OTU水平的NMDS分析

    T0、T5、T10、T15、T20、T25、T30分别指未种植硒砂瓜处理及连作5年、10年、15年、20年、25年和30年处理。

    Figure  5.  NMDS analysis of soil fungi based on OTU level in different continuous cropping years of selenium-melon

    T0, T5, T10, T15, T20, T25, and T30 refer to the treatments of non-planting and continuous cropping for 5, 10, 15, 20, 25 and 30 years of selenium melon, respectively.

    表  1  硒砂瓜连作对土壤理化性质的影响

    Table  1.   Effects of selenium-melon continuous cropping on soil physical and chemical properties

    处理
    Treatment
    pH 有机质
    Organic matter
    (g·kg-1)
    全氮
    Total N
    (g·kg-1)
    速效磷
    Available P
    (mg·kg-1)
    全磷
    Total P
    (g·kg-1)
    速效钾
    Available K
    (mg·kg-1)
    全钾
    Total K
    (g·kg-1)
    碱解氮
    Alkali-hydrolysis N
    (mg·kg-1)
    T0 8.07±0.26c 9.17±0.41ab 0.41±0.04ab 7.90±0.91b 0.61±0.03ab 122±22.07ab 24.10±0.69a 29.87±5.94a
    T5 8.22±0.10bc 9.45±0.43a 0.53±0.12a 18.90±4.52a 0.90±0.25a 141±7.77a 23.40±0.66ab 34.83±3.38a
    T10 8.51±0.01ab 9.22±1.52ab 0.47±0.12ab 4.00±0.99b 0.60±0.08b 112±4.24ab 24.50±1.69a 21.95±8.69a
    T15 8.67±0.06a 10.32±0.58a 0.45±0.02ab 5.00±2.00b 0.66±0.05ab 138±10.07a 23.90±0.26ab 28.80±4.00a
    T20 8.58±0.04ab 9.17±1.44ab 0.43±0.02ab 6.50±1.54b 0.64±0.03ab 136±8.18a 23.77±0.26ab 27.13±4.09a
    T25 8.49±0.10ab 9.32±1.11ab 0.42±0.04ab 2.47±1.10b 0.65±0.07ab 119±2.31ab 23.67±0.23ab 28.10±5.64a
    T30 8.30±0.23bc 7.45±0.04b 0.33±0.02b 3.50±0.42b 0.64±0.01ab 100±15.56b 21.60±2.12b 29.20±7.60a
    T0、T5、T10、T15、T20、T25、T30分别指未种植硒砂瓜处理及连作5年、10年、15年、20年、25年和30年处理。T0, T5, T10, T15, T20, T25, and T30 refer to the treatments of non-planting and continuous cropping for 5, 10, 15, 20, 25 and 30 years of selenium melon, respectively.
    下载: 导出CSV

    表  2  硒砂瓜连作土壤真菌Alpha多样性指数

    Table  2.   Alpha diversity indexes of soil fungal under selenium-melon continuous cropping

    样本
    Sample
    Shannon指数
    Shannon Index
    辛普森指数
    Simpson Index
    Ace指数
    Ace Index
    Chao指数
    Chao 1 Index
    T0 2.61±1.41 0.28±0.17 346.65±34.07 347.81±43.78
    T5 1.97±0.79 0.37±0.19 250.83±48.33 252.55±47.45
    T10 3.03±1.05 0.17±0.13 408.25±171.99 411.57±174.03
    T15 3.50±0.43 0.11±0.05 496.99±36.03 506.77±38.04
    T20 2.92±1.46 0.22±0.02 374.92±42.56 361.88±63.91
    T25 1.91±0.81 0.32±0.19 194.89±87.11 192.85±86.26
    T30 2.58±0.87 0.17±0.11 202.22±24.55 207.53±14.91
    T0、T5、T10、T15、T20、T25、T30分别指未种植硒砂瓜处理及连作5年、10年、15年、20年、25年和30年处理。T0, T5, T10, T15, T20, T25, and T30 refer to the treatments of non-planting and continuous cropping for 5, 10, 15, 20, 25 and 30 years of selenium melon, respectively.
    下载: 导出CSV

    表  3  土壤真菌优势菌(属水平)丰度与土壤理化性质的相关性

    Table  3.   Correlation between abundance of soil fungal dominant bacteria (genus level) and soil physicochemical properties

    优势属
    Dominant genera
    pH 有机质
    Organic matter
    全磷
    Total P
    全钾
    Total K
    碱解氮
    Alkali-hydrolysis N
    速效钾
    Available K
    速效磷
    Available P
    被孢霉属Mortierella 0.159 0.066 -0.022 -0.314 -0.079 -0.283 -0.395
    绿僵菌属Metarhizium 0.326 0.354 -0.404 0.515* -0.399 0.107 0.111
    假霉样真菌属Pseudallescheria -0.065 0.187 0.486* 0.008 0.402 0.636** 0.417
    镰刀菌属Fusarium 0.105 0.058 -0.597** 0.372 -0.610** -0.267 -0.208
    青霉属Penicillium 0.438* -0.068 -0.183 0.143 -0.052 -0.027 -0.079
    Unclassified_Ascomycota 0.180* 0.379 0.088 -0.029 -0.198 0.091 -0.384
    毛壳属Chaetomium 0.318 0.219 0.206 0.129 0.050 0.487* 0.160
    **P < 0.01, *P < 0.05.
    下载: 导出CSV
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出版历程
  • 收稿日期:  2018-08-07
  • 录用日期:  2018-11-26
  • 刊出日期:  2019-04-01

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