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高通量测序技术在微生物分子生态学研究中的应用

王新珍 王凤花 孙瑞波 刘彬彬

王新珍, 王凤花, 孙瑞波, 刘彬彬. 高通量测序技术在微生物分子生态学研究中的应用[J]. 中国生态农业学报(中英文), 2018, 26(10): 1593-1600. doi: 10.13930/j.cnki.cjea.180695
引用本文: 王新珍, 王凤花, 孙瑞波, 刘彬彬. 高通量测序技术在微生物分子生态学研究中的应用[J]. 中国生态农业学报(中英文), 2018, 26(10): 1593-1600. doi: 10.13930/j.cnki.cjea.180695
WANG Xinzhen, WANG Fenghua, SUN Ruibo, LIU Binbin. Application of high-throughput DNA sequencing in microbial ecology[J]. Chinese Journal of Eco-Agriculture, 2018, 26(10): 1593-1600. doi: 10.13930/j.cnki.cjea.180695
Citation: WANG Xinzhen, WANG Fenghua, SUN Ruibo, LIU Binbin. Application of high-throughput DNA sequencing in microbial ecology[J]. Chinese Journal of Eco-Agriculture, 2018, 26(10): 1593-1600. doi: 10.13930/j.cnki.cjea.180695

高通量测序技术在微生物分子生态学研究中的应用

doi: 10.13930/j.cnki.cjea.180695
基金项目: 

国家重点研发计划项目 2016YFD0800100

国家自然科学青年基金项目 41601511

河北省优秀青年基金项目 D2017503022

详细信息
    作者简介:

    王新珍, 主要研究领域为环境微生物学。E-mail:xzwang@sjziam.ac.cn

    通讯作者:

    刘彬彬, 主要研究领域为微生物分子生态学和生物信息学。E-mail:binbinliu@sjziam.ac.cn

  • 中图分类号: Q93-31;Q938.1

Application of high-throughput DNA sequencing in microbial ecology

Funds: 

the National Key Research and Development Project of China 2016YFD0800100

the National Natural Science Foundation of China 41601511

Foundation for Distinguished Young Scholars of Hebei Province D2017503022

More Information
  • 摘要: 微生物在众多的自然和人工生态系统中发挥着核心的作用,但能够被培养分离的微生物在大部分生态系统中只占极少一部分,极大地限制了人们对微生物组成、功能及其潜在应用的认识。分子生物学方法,尤其是高通量测序技术应用到微生物生态学研究中,为认识微生物多样性、群落结构组成及其生态功能提供了有利手段。高通量测序作为一种新兴的免培养分子生物学技术,具备检测快速、准确、信息全面丰富等特点。随着高通量测序技术的不断升级换代,测序通量、读长和准确度的不断提升以及成本的大幅下降,该技术在过去十几年间被迅速应用于土壤、水体和肠道等微生物区系的研究中。本文简述了基于高通量测序技术的PCR产物测序技术和宏基因组学测序技术的原理、发展历程、数据分析方法与应用,以及宏基因组学测序技术在病毒学领域的应用,以期为微生物分子生态学研究提供参考。
  • 图  1  典型的宏基因组研究流程图(虚线表示可省略的步骤)[17]

    Figure  1.  Flow diagram of a typical metagenome project (dashed arrows indicate steps that can be omitted)[17]

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  • 收稿日期:  2018-07-15
  • 录用日期:  2018-08-05
  • 刊出日期:  2018-10-01

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