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黄瓜与西芹间作对黄瓜土壤真菌ITS多样性分析

秦立金 于田田 王佳明 高寅彪 王守政 李铮 云兴福

秦立金, 于田田, 王佳明, 高寅彪, 王守政, 李铮, 云兴福. 黄瓜与西芹间作对黄瓜土壤真菌ITS多样性分析[J]. 中国生态农业学报(中英文), 2019, 27(4): 529-536. doi: 10.13930/j.cnki.cjea.180484
引用本文: 秦立金, 于田田, 王佳明, 高寅彪, 王守政, 李铮, 云兴福. 黄瓜与西芹间作对黄瓜土壤真菌ITS多样性分析[J]. 中国生态农业学报(中英文), 2019, 27(4): 529-536. doi: 10.13930/j.cnki.cjea.180484
QIN Lijin, YU Tiantian, WANG Jiaming, GAO Yinbiao, WANG Shouzheng, LI Zheng, YUN Xingfu. Soil fungal ITS diversity in cucumber-celery intercropping[J]. Chinese Journal of Eco-Agriculture, 2019, 27(4): 529-536. doi: 10.13930/j.cnki.cjea.180484
Citation: QIN Lijin, YU Tiantian, WANG Jiaming, GAO Yinbiao, WANG Shouzheng, LI Zheng, YUN Xingfu. Soil fungal ITS diversity in cucumber-celery intercropping[J]. Chinese Journal of Eco-Agriculture, 2019, 27(4): 529-536. doi: 10.13930/j.cnki.cjea.180484

黄瓜与西芹间作对黄瓜土壤真菌ITS多样性分析

doi: 10.13930/j.cnki.cjea.180484
基金项目: 

国家自然科学基金项目 31160100

2018年内蒙古自治区科技重大专项和内蒙古自治区应用技术研究与开发项目 201602050

详细信息
    作者简介:

    秦立金, 研究方向为设施蔬菜土传病害生物防治与土壤修复。E-mail:1597355169@qq.com

    通讯作者:

    云兴福, 研究方向为高寒地区蔬菜栽培与生理。E-mail:yxf5807@163.com

  • 中图分类号: S476

Soil fungal ITS diversity in cucumber-celery intercropping

Funds: 

the National Natural Science Foundation of China 31160100

Major Science and Technology Project of Inner Mongolia Autonomous Region in 2018, Application Technology Research and Development Project of Inner Mongolia Autonomous Region 201602050

More Information
  • 摘要: 为探索黄瓜与西芹间作后对黄瓜土壤真菌多样性变化规律的影响,本试验以黄瓜与西芹间作种植模式为处理,黄瓜单作和西芹单作种植模式为对照,进行不同处理土壤ITS真菌群落多样性高通量测序分析。结果表明:黄瓜与西芹间作土壤真菌Alpha多样性指数降低,Observed species指数、Shannon指数、Chao1指数和Simpson指数与对照相比均达到最低值,但差异均不显著(P>0.05)。在门分类水平上,共检测到5个菌门,其中,子囊菌门(Ascomycota)、接合菌门(Zygomycota)和担子菌门(Basidiomycota)为3个主要菌门;黄瓜与西芹间作处理前3个菌门所占比例最高,达95.50%。在属分类水上,共检测到329属,相对丰度比例为前12的菌属占所有检测出菌属的49.4%;黄瓜与西芹间作处理检测的菌属所占比例最高,达50.72%,其次为黄瓜单作和西芹单作,分别为50.47%和47.17%。因此,黄瓜与西芹间作处理改变了黄瓜土壤真菌群落的结构和组成,丰富了黄瓜土壤真菌的群落多样性,为以后设施黄瓜连作土壤微生态环境改善和修复提供科学依据。
  • 图  1  黄瓜与西芹间作土壤的真菌OUT分布Venn图

    JZ:黄瓜与西芹间作; HD:黄瓜单作; XD:西芹单作。

    Figure  1.  Venn graph of soil fungi OUT distribution under cucumber and celery intercropping

    JZ: cucumber and celery intercropping; HD: cucumber monoculture; XD: celery monoculture.

    图  2  黄瓜与西芹间作土壤真菌门水平的群落组成

    JZ:黄瓜与西芹间作; HD:黄瓜单作; XD:西芹单作。

    Figure  2.  Community composition of soil fungi at phylum level under cucumber and celery intercropping

    JZ: cucumber and celery intercropping; HD: cucumber monoculture; XD: celery monoculture.

    图  3  黄瓜与西芹间作土壤门水平上真菌物种分类热图

    JZ:黄瓜与西芹间作; HD:黄瓜单作; XD:西芹单作。

    Figure  3.  Heatmap describing fungi species classification at phylum level of soil under cucumber and celery intercropping

    JZ: cucumber and celery intercropping; HD: cucumber monoculture; XD: celery monoculture.

    图  4  黄瓜与西芹间作处理土壤属水平上真菌物种分类热图

    JZ:黄瓜与西芹间作; HD:黄瓜单作; XD:西芹单作。

    Figure  4.  Heatmap describing fungi species classification at genus level of soil fungi under cucumber and celery intercropping

    JZ: cucumber and celery intercropping; HD: cucumber monoculture; XD: celery monoculture.

    表  1  黄瓜与西芹间作土壤真菌的测序数据质量结果

    Table  1.   Quality results of sequencing data of soil fungi under cucumber and celery intercropping

    处理
    Treatment
    原始数据Raw data 有效数据Valid data 有效百分
    比Valid (%)
    Q20 (%) Q30 (%) GC (%)
    Tag Base (M) Tag Base (M)
    西芹单作
    Celery monoculture
    109 237.67 51.78 108 839.67 28.43 99.61 99.29 97.85 57.42
    黄瓜单作
    Cucumber monoculture
    93 919.67 35.04 73 673.00 19.31 99.65 99.06 97.22 58.16
    黄瓜与西芹间作
    Cucumber and celery intercropping
    62 416.00 29.59 61 831.00 15.94 98.94 99.29 97.82 58.34
    下载: 导出CSV

    表  2  黄瓜与西芹间作土壤的真菌Alpha多样性指数

    Table  2.   Alpha diversity indexes of soil fungi community under cucumber and celery intercropping

    处理Treatment Observed species指数
    Observed species index
    Shannon指数
    Shannon index
    Chao1指数
    Chao1 index
    Simpson指数
    Simpson index
    西芹单作Celery monoculture 1 135.67±25.71a 6.85±0.06a 1 361.61±36.73a 0.97±0.003a
    黄瓜单作Cucumber monoculture 1 033.00±61.65a 6.60±0.08a 1 207.83±72.43a 0.97±0.000a
    黄瓜与西芹间作
    Cucumber and celery intercropping
    955.67±29.91a 6.40±0.18a 1 193.93±56.86a 0.96±0.007a
    小写字母相同表示不同处理间差异不显著(P > 0.05)。Data with same lowercase letters are not significantly different at 0.05 level.
    下载: 导出CSV

    表  3  黄瓜与西芹间作土壤属水平真菌的群落组成

    Table  3.   Community composition of soil fungi at genus level under cucumber and celery intercropping

    菌属
    Fungal genus
    Reads 比例Ratio (%)
    平均
    Average
    西芹单作
    Celery monoculture
    黄瓜单作
    Cucumber monoculture
    黄瓜与西芹间作
    Cucumber and celery
    intercropping
    Wardomyces 42 349 6.00 4.80 5.67 7.27
    光黑壳属Preussia 42 253 6.00 4.50 6.50 7.03
    丝孢菌属Scedosporium 38 243 5.40 6.17 5.10 4.40
    被孢霉属Mortierella 29 151 4.10 4.77 3.93 2.80
    枝孢属Cladosporium 25 145 3.50 2.60 4.03 5.47
    腐质霉属Humicola 21 848 3.10 3.43 3.10 2.43
    漆斑菌属Myrothecium 21 625 3.00 2.13 3.63 4.23
    毛壳属Chaetomium 20 034 2.80 2.70 3.80 1.90
    Davidiella 15 308 2.20 1.90 2.13 2.67
    柄孢壳菌属Podospora 11 999 1.70 1.67 1.83 2.27
    链格孢属Alternaria 8 865 1.20 1.30 0.67 1.87
    Cystilepiota 7 463 1.10 1.53 0.10 0.03
    其他属Others 360 054 50.60 52.83 49.28 49.53
    未鉴别/未分类属Unclassed/unidentified 66 176 9.30 9.67 10.23 8.10
    下载: 导出CSV
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出版历程
  • 收稿日期:  2018-05-19
  • 录用日期:  2018-12-14
  • 刊出日期:  2019-04-01

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