泰国产方斑东风螺养殖群体遗传多样性的RAPD分析
Genetic diversity analyse of Thailand Babylonia areolata with RAPD markers
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摘要: 用随机扩增多态性DNA(RAPD)技术对泰国产方斑东风螺养殖群体的遗传多样性进行检测，从100个随机引物中筛选出21个引物对方斑东风螺的DNA进行扩增，结果表明：21个引物共检测到222条清晰且重复性好的条带，每个引物可扩增出4～16条带，分子量在200～2200bp之间，其中多态位点为156个，占70.27%；群体的Shannon多样性指数为0.2818，Nei基因多样性指数为0.2491；个体间最大遗传距离为0.291，最小遗传距离为0.066。通过与其他贝类遗传多样性的研究结果比较，可初步判断泰国产方斑东风螺养殖群体的遗传多样性比较丰富。Abstract: Random Amplified Polymorphic DNA(RAPD)technique was applied to detect DNA polymorphism in 20 individuals of an artificially grown population of Babylonia areolata imported from Thailand.21 random primers were used from which a total of 222 bands were generated.Each primer used in the detection has 4～16 bands,with DNA fragment lengths ranging from 200 to 2200bp.Observed total amount and percentage of the polymorphic fragments are 156 and70.27%,and the Shannon and Nei index of phenotypic and gene diversity of raised population are 0.2818 and 0.2491 respectively.Genetic distance among individuals ranges from 0.066 to 0.291.Comparing genetic diversity of B.areolata with that of other shellfish reveals a hefty genetic diversity of B.areolata.
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