玉米苞叶中总黄酮提取及其体外抗氧化性能的研究

Extraction of corn-bract flavonoid and related antioxidative activity

    摘要
  • 摘要: 运用超声波技术提取玉米苞叶中的总黄酮，用亚硝酸钠-硝酸铝比色法测定总黄酮含量，得出玉米苞叶中总黄酮提取的最佳工艺条件：超声波功率 700W，温度60℃，料液比(g/mL)1∶50，乙醇浓度为60%，提取时间为35min，玉米苞叶中总黄酮最大提取率为1.225%。体外抗氧化试验表明：玉米苞叶中的总黄酮对超氧阴离子自由基、羟基自由基清除能力较好，其IC50分别为 1.5μg/mL左右和0.14mg/mL，清除能力均高于Vc；对DPPH自由基体系的清除率达到50%时，玉米苞叶中总黄酮的 IC50为0.2mg/mL左右，Vc的IC50为0.075mg/mL，说明玉米苞叶中的总黄酮清除DPPH自由基的能力低于Vc，但在所选浓度剂量范围内，其最大清除率高于Vc。试验表明，玉米苞叶中的总黄酮是一种较好的天然自由基清除剂。

     

    Abstract: The craft of corn-bract flavonoid extraction with ultrasonic and its content measurement with colorimetry was conducted and the best distillation conditions found to be at 700W of ultrasonic power,extraction temperature of 60℃，material proportion of 50g/mL,ethanol concentration of 60%,extraction time of 35 min.The highest total flavonoid rate gets 1.225%.Antioxidative test results show good performance of flavonoid scavenging capacity in both superoxide radical systems and hydroxyl radical systems.Free radical scavenging capacity, IC50 flavonoids, to superoxide radical system and hydroxyl radical are respectively 1.5μg/mL and 0.14mg/mL and stronger than vitamin C(VC).In DPPH system,IC50 flavonoid is 0.2mg/mL and IC50 VC is 0.075mg/mL.However,within selected dosage,the highest scavenging capacity of flavonoids outweighs that of VC.The tests show the suitability of flavonoid as good natural radical scavenger.

     

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