Abstract: Selecting Cry1Ab gene-carrying rice germplasm “Kemingdao” as the insect-resistant donor,we transferred the target gene into two elite rice restorer lines“R6547”and “R818” through crossing and backcrossing. The hybrid combinations derived from two transgenic restorer lines show significant heterosis that do overcome the defects of undesirable agronomic traits and weak prophase development capabilities. Results of testing the Cry1Ab protein indicate that the target gene expresses strongly and provides good resistance to stripe stem borers (Chilo suppressalis) and leaf folders (Cnapha locrocis medinalis) in hybrid combinations.