Abstract: Muskmelon, soillessly cultured with organic substrate was used to study fertilization effect on yield, nutrient absorption and distribution, and utilization rates of N, P and K. Results indicate an obvious increase in yield and nutrient utilization rate of muskmelon. But nutrient utilization rate decreases once fertilization ratio approaches excessive levels. Muskmelon yield is highest in the second treatment where the amount of applied fertilizer is calculated by using the following equation:Fertilizer amount=(fertilizer for desired output-organic substrate nutrient content)/nutrient uptake ratio.