Abstract: Field experiment was conducted to research the effect of manure and chemical fertilizer on the number of nitrogen transferrer edaphon(e.g.aerobic nitrogen-fixing bacteria, ammonifiers,nitrite bacteria and denitrifying bacteria) in black soil ecosystems.Results show that application of manure increase the number of aerobic nitrogen-fixing bacteria, ammonifiers and denitrifying bacteria, while chemical fertilizers reduce the number of aerobic nitrogen-fixing bacteria in black soils, increase the number of denitrifying bacteria.Otherwise combination of equally proportioned manure and chemical fertilizer promotes aerobic nitrogen-fixing bacteria, nitrite bacteria and denitrifying bacteria activities.