Abstract: A pot experiment was conducted in a completely randomized block design to study the effect of different phosphate-dissolving groups of microorganism on soil nutrient and enzymatic activity of rape in the north lime soil. Results indicate that all the phosphate-dissolving groups of microorganism can increase soil available nutrient and soil enzymatic activity in the different treatments in rape breeding period.By increasing the level of phosphorus, soil phosphatase activity drops. Soil phosphatase activity is higher with bacterial manure application than without bacterial manure. There are significant differences in urase activity in rape seedlings at different levels of phosphorus.