温度对淡紫拟青霉NH-PL-03菌株培养特性及毒力的影响

Effect of temperature on Paecilomyces lilacinus culture characteristics and its toxicity to Ditylenchus destructor

    摘要
  • 摘要: 试验测定了淡紫拟青霉NH-PL-03菌株在不同温度条件（15～35℃）下培养过程中生长特性（OD值、pH值、菌丝干重、孢子量、基质消耗）的变化趋势及对线虫的致死率，系统研究温度对淡紫拟青霉生长的影响，结果表明：温度不仅影响菌体的生长，且影响次生代谢物的毒力。在15～30℃范围内，菌体生长、孢子生成及基质消耗（C、N）速率随温度升高而升高；25℃为菌体生长的最合适温度，培养第5d进入快速增长期，第9d菌丝干重达到1.08g，第10d菌液孢子量达到401.2万个/mL；30℃时总体毒力最高，第7d培养液对茎线虫的致死率达到64.60%，其次是25℃和20℃，最高致死率分别达到56.17%和37.21%；15℃ 最高致死率仅为24.71%。说明在一定范围内，温度上升有利于毒力产物生成。

     

    Abstract: In this paper,the effect of temperature on Paecilomyces lilacinus growth was investigated.The growth index for OD,pH,mycelia dry weight,and spore number and their toxic effect on nematodes at different temperatures (15～35℃）were analyzed.Results show that temperature is a key factor of fermentation.Paecilomyces lilacinus exhibits normal growth within 20～30℃，optimum mycelia growth and sporulation occur at 25℃,mycelia biomass reaches 1.08g in 9 days,spore number reaches 4.012×106/mL in 10 days.Between 15～30℃，higher temperatures can increase C and N consumption and toxin production.At 30℃,cultured 7d,the toxicity to Ditylenchus destructor can reach 64.60%.

     

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