盐胁迫下两种基因型冬小麦萌发过程中钠离子吸收及对碳、氮代谢的影响

Sodium uptake and carbon/nitrogen metabolism by different winter wheat genotypes during germination under salt stress

  • 摘要: 采用两种基因型冬小麦种子为试验材料,研究了盐胁迫对小麦种子萌发过程中Na+的吸收和累积及其对C、N代谢的影响及外源Ca(NO3)2和CaCl2对小麦萌发过程中盐害的缓解效应。结果表明,150mmol/L NaCl胁迫明显抑制小麦种子的萌发。种子萌发过程中,“小偃6号”的耐盐性优于“陕229”。外源钙对两种小麦盐胁迫效应的缓解作用不同。6mmol/L钙 离子可明显减轻“陕229”的盐害作用,对“小偃6号”作用不大。盐胁迫对种子萌发过程中的蛋白酶和淀粉酶活性具有明显抑制作用。盐胁迫下,“小偃6号”种子的蛋白酶和淀粉酶活性明显高于“陕229”。钙离子对盐胁迫下小麦萌发过程胚乳内蛋白酶的活性有较大调节作用,对淀粉酶活性影响较小。盐胁迫下小麦种子中Na+含量显著增加,K/ Na比显著降低。钙离子对盐胁迫下小麦种子中Na+累积的影响较大,盐胁迫和外源钙处理对K+含量 影响较小。

     

    Abstract: The effect of salt stress on sodium uptake and accumulation,carbon and nitrogen metabolism and the ameliorative effect of calcium on wheat seed germination were studied.Results indicate that wheat seed germination is inhibited by 150mmol/L NaCl.“Xiaoyan6”is more tolerant than “Shaan229” during wheat germination.The ameliorative effect of exogenous calcium on the germination of the two wheat genotypes is different.Salt injury on “Shaan229” is significantly reduced by the addition of 6 mmol/L Ca2+,but calcium has no ameliorative effect on “Xi aoyan6”.Salt stress can decrease proteinase and amylase activities during wheat germination. “Xiaoyan6” enzyme activity is higher than that of “Shaan229”.Proteinase activity is improved by calcium while amylase activity is little affected by calcium.Na+ content in wheat seeds increases remarkably while K/Na ratio decreases significantly under salt stress.Na+ content of wheat seed is affected by exogenous calcium,but K+ content is little affected under 150 mmol/L NaCl and calcium.

     

/

返回文章
返回