高毒效杀蚜球孢白僵菌菌株筛选

Beauveria bassiana isolate selection against aphids

  • 摘要: 试验研究球孢白僵菌Beauveria bassiana 4菌株“Bb7001”、“Bb7004”、“Bb8001”、“Bb4014”在21℃下对桃蚜Myzus persicae (Sulzer)的侵染致病力及对桃蚜生殖力和种群参数的影响,结果表明:在21℃下,球孢白僵菌对桃蚜的侵染致病力和对其生殖力及种群参数的影响在总体上基本一致,但三者在4菌株间也有一定差异,强弱依次为“Bb7001”>“Bb7004”>“Bb4014”>“Bb8001”;桃蚜接种不同浓度4菌株后在第3~5d达到死亡高峰期,lgLC50分别为3.67、4.34,4.52和4.48;接种球孢白僵菌4菌株106(孢子)/mL后,桃蚜成蚜的生殖力在第3d均与对照组桃蚜生殖力有显著差异,10d内日均产仔与对照蚜相比下降30.06%~50.00%;球孢白僵菌4菌株对桃蚜净生殖力有极显著降低作用,对内禀增长力也有一定的降低作用,接种4菌株后桃蚜净生殖力下降61.90%~72.73%,内禀增长力降低13.04%~41.58%,但对世代历期几乎没有影响。菌株“Bb7001”显示了较高的杀蚜毒效,可考虑作为防治桃蚜的首选菌株进行开发。

     

    Abstract: Fungal pathogenicity and fecundity, and effects on population parameters and fecundity of aphids of 4 isolates of Beauveria bassiana were studied (under 21℃, 85% relative humidity). The results show a general consistency between infection mortality to aphids and effect on the reproduction and fecundity of the aphids, but a significant difference among the 4 isolates exists. On the whole, the observed infection rank order of performance of the isolates is “Bb7001”>“Bb7004”>“Bb4014”>“Bb8001”. The aphids' mortality is highest 3~5 days after inoculation of the 4 Beauveria bassiania isolates “Bb7001”,“Bb7004”,“Bb4014” and “Bb8001” with lgLC50 at 3.67,4.34,4.52 and 4.48. The fecundity of aphids is significantly different from the control after inoculated with the isolates in 106 conidia per mL for 3 days. 4 isolates show significant inhibition for reproduction. Within 10 days, the birth rate of aphid offspring decreases by 30.06%~50.00%; the net reproductive rate decreases by 61.90%~72.73%; and the intrinsic increasing rate decreases by 13.04%~41.58%. Among all the isolates, the “Bb7001”exhibits the most significant effect on the green peach aphids and is the first isolate to develop.

     

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