苗期叶面施硅对生姜叶片膜脂过氧化及保护酶活性的影响

Effect of spraying silicon in the seedling stage of ginger on membrane fluid peroxidation and leaf defense enzyme activity

  • 摘要: 在生姜发棵期研究叶面喷施不同浓度硅酸钠对生姜叶片膜脂过氧化及保护酶活性的影响,结果表明:适量浓度的Si可降低姜叶片中的丙二醛(MDA)含量和相对电导率(REC),保护植物细胞膜的完整性,但过高浓度及过量的Si反而使MDA含量和REC升高;经过3次处理后,生姜叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)活性比对照均有不同程度提高,但以第1次处理效果最为显著,第2次、第3次处理后酶活性上升幅度减缓,高浓度的Si反而造成酶活性的下降;叶面喷施Si的浓度以500~1000mg/L效果较好,能防止生姜叶片的膜脂过氧化,增强叶片保护酶的活性。

     

    Abstract: Different concentrations of sodium silicate were sprayed on ginger leaves at seedling stage.Its results show that appropriate concentration of silicon reduces malondialdehyde(MDA)content and relative electric conductivity(REC) which protects cell membrane integrality.On the other hand,excessive silicon increases the content of MDA and REC.Detail analysis show that sodium silicate treatment increases the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and phenyllamine ammonialyase(PAL) to varying degrees.The effects of the first treatment are remarkable,but the activities of enzymes gradually increases after second and third treatments.Thus it is concluded that excessive silicate decreases the activities of SOD,POD,CAT,and PAL.Silicon concentration of 500~1000mg/L produces desirable effects.It raises the antioxidant ability of ginger leaves and increases the activities of defense enzymes.

     

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