Abstract: The effects of DMSO （Dimethylsulfoxide）,in which wheat seeds were soaked,on the cold-resistance of seedlings were studied. The results show that DMSO treatment can enhance the activity of SOD and POD, raise the content of chlorophyll and content of soluble protein,and the vitality of the root system, reduce the content of malondialdehyde （MDA）and the cell electrolyte leakage of the seedlings.DMSO also can enhance the plant height,dry weight and the ratio of root to shoot of wheat seedlings under cold stress.The suitable concentration of DMSO is 2～5g/kg.