利用植物体细胞无性系变异技术选育高粱恢复系

A restorer line ‘011’for hybrid sorghum production developed by using somaclonal variation technique

  • 摘要: 试验研究以高粱恢复系“0-30”幼穗作外植体,用10Gy60Co辐照处理,离体培养得到89株再生苗(R0)。R1代株系27.9%出现7种可见性状的变异,从第2代(R1)中筛选出稳定或分离的优良变异株,经多代选择最终选育出高粱恢复系“011”,用其配制的杂交种“7050A/ 011”产量高且抗病性强。

     

    Abstract: The young inflorescences collected from ‘0-30’, the male parent of ‘Shenza 5’,were exposed to 60Co-γrays at a dose of 10GY, then in-vitro cultured on MS media. A total of 89 R0 regenerants were obtained. Solid and segregating somaclonal variations in seven kinds of visual traits were seen in R1 generation with a variation frequency of 27.9%. Elite variants were isolated from the R1 solid and segregating population and crossed to sorghum male sterile lines. A somaclonal restorer called ‘011’ were selected out. The hybrid combination ‘7050A/011’ was featured by its high production and high antifungal.

     

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