Abstract: Foreign triple genes(Bt＋CpTI＋GNA) resistant to cotton insects have been introduced into conventional cotton cultivars by agrobacterium tumefaciens mediated transformation and the regenerated plants have been obtained. The results of molecular detection indicate that the foreign genes have been transferred into cotton plants. The progeny materials of transgenic plants are detected by insect-resistance. Up to now, the F₈ has been got after the selection in the fields. The results of PCR molecular detection, marker genes and foreign target-gene with insect-resistance are quite regular. Some of their progenies' segregations coincide with the Mendel's regularity of segregation, but some deviate, and their segregation laws in lower transgenic generation materials are vague while those in high generation materials are stabler.