镉诱导的茶苗茎尖核酸代谢与细胞超微结构变化研究

Metabolism of nucleic acid and changes of cell ultrastructure in stem apex o tea seedling induced by Cd2+

  • 摘要: 试验研究茶苗经不同浓度Cd2+培养液培养12d结果表明,0.05mmol/L浓度Cd2+对茶苗相对生长量无明显影响,≥0.10mmol/L浓度Cd2+时随处理浓度增加和时间延长,茶苗生长受抑程度逐渐加大。培养12d时0.05mmol/L浓度Cd2+处理茶苗细胞内核糖体数量和核酸含量均高于对照,超过此浓度的处理其核糖体大量消亡,核酸含量显著降低,其中DNA含量变幅小于RNA。0.0~0.10mol/L浓度Cd2+处理RNase活性随浓度增加而升高,>0.25mmol/L浓度Cd2+处理RNase活性则随浓度增加而降低。电镜观察结果表明轻度Cd2+毒害表现为核仁解体,染色质凝聚,核膜间隙扩大,核染色质进入核膜间隙,严重者出现核变形收缩,核膜破裂,核染色质扩散降解最终核解体。

     

    Abstract: Tea seedlings were treated for 12 days by different concentrations of culture solution with Cd2+. The results show that the bbiomass of the seedlings is not affected when Cd2+2+ concentration and treatment time when Cd2+ concentration is ≥0.10 mmol/L.The content of nucleic acid increases at Cd2+ level of 0.05mmol/L and decreases at Cd2+ levell ranging from 0.10mmol/L to 0.50mmol/L. RNA is more sensitive toCd2+ stress compared with DNA. The Rnase activity increases at Cd2+ level ranging from 0.00mmol/L to 0.10mmol/L and decreases at Cd2+ level ranging from 0.25mmol/L to 0.50mmol/L. The nucleus ultrastructure changes progressively with the increase of Cd2+ concentration. When seedlings are treated with 0.05mmol/L Cd2+, the nucleolus disintegrates and the chromatin condenses, the perinuclear spaces are slightly dilated. The deeply expanded perinuclear membrane contains nuclear materials at Cd2+ level of 0.10mmol/L. More serious damage is shown in the nucleus with higher Cd2+ concentrations, such as deformation of nucleus, shrinking oof nucleus, scattering of condensed chromatin and eventually disruption of nuclear membrane.

     

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