Abstract: The effect of 4 kinds of antibiotics (Cb, Cef, Hyg, Kan) on the regeneration of sorghum shoot and the establishment of sorghum regeneration system were tested. The results indicate that the MS with glycine, Fe-EDTA and vitamin C is the best regeneration medium for sorghum shoot. Meanwhile, 250mg/L of agrobacterium is the most suitable antibiotic concentration for Cb in genetical transfrmation of sorghum shoot and the screening tactics of anti-fungus and transformed tissue are established.