Abstract: RNA silencing occurs in plants is conceived as a natural antiviral defense system that is activated as a response to double-stranded RNA (dsRNA) formed during virus replication.To compensate for the disadvantages on RNA silencing by using dsRNA to specifically interfere with virus infection in transgenic plants,several approaches of obtaining dsRNA used for virus-resistance have been developed which differ from strategies based on transgenic expression of RNAs but still rely on RNA silencing as a means to achieve pathogen-derived resistance (PDR).In this paper,the dsRNA produce strategies for RNA silencing,their applications for virus resistance,disadvantages and perspectives are reviewed.