Abstract: Effects of medium,season and biological measures on the rooting,growth and chlorophyll content in cutting plant of Taxus chinensis var.mairei were studied.The results show that the mixture of river sand and pearl-rock at the ratio of 8∶2 is the best medium of seedling bed.The green plant rate,rooting rate and survival rate are 86.0%,84.3% and 81.7%,respectively,being significantly higher than those of the other three media.Then,the mixture of river sand and fired-soil is the second,the yellow soil is the third,and the river sand is the worst.The efficiencies of season and biological measures show that the fourth season in a year is the most optimal period for cutting propagation and significant differences are observed between seasons.Also,the cultivatioin of soybean along with cutting of Taxus is better than without cultivation of soybean and the significant difference is observed between them.