Abstract: The studies on the enzyrfte activities and fertility change of soils by a long-term located utilization of different fertilizers in wheat-maize rotation district show that the activities of unease，alkaline phosphatase，invertase and hydrogen peroxidase soils Call be increased by a long-term fertilization，and the effect of inorganic fertilizer combined with organic manure is better than that of only inorganic fertilizer，and the activity of soil enzyme can not be increased in fallow field af-ter fertilization，showing that fertilization(inorganic and organic fertilizer)and crop rhizctaxis both can increase the activity of soil enzyme，and the activity of soil enzyme are positively correlated with crop yield(soil fertility)．