应用RAPD技术研究4种鲍的亲缘关系

Study on genetic relationship among 4 kinds of abalone using RAPD technique

  • 摘要: 应用RAPD技术研究4种鲍的亲缘关系结果表明,4种鲍群体中20个有效引物共扩增出538条DNA带,平均每个引物扩增的条带数为26.9;扩增的多态性条带数136,平均每个引物扩增的多态性条带数为6.8;多态位点百分数为25.3%。盘鲍群体和皱纹盘鲍群体之间遗传距离与遗传一致度分别为0.28和0.72,杂色鲍群体和九孔鲍群体之间遗传距离与遗传一致度分别为0.32和0.68。聚类分析把盘鲍群体和皱纹盘鲍群体聚为1组,二者亲缘关系较近;杂色鲍群体和九孔鲍群体聚为1组,二者亲缘关系也较近。

     

    Abstract: RAPD technique is used tO study the genetic relationship among 4 kinds of aba~ne.The results show that total 538 DNA bands are scoped by 20 effective primers in 4 kinds of abalone,of which 136 DNA bands are reprod ucibly polymorphic,and the percentage of polymorphic products is 25.3% ;the genetic distance and genetic identity between Haliotis discus discus and H .discus hannai are 0.28,0.72 respectivdy,those between H .diversicolor diversicolor and H .diversicolor supertexta are 0.32,0.68 respectively.In the results of cluster analysis,H .discus discus and H .discus hannai are pooled into one group,they both are genetically closer;H.diversicolor diversicolor and H .diversicolor SUpertexta are pooled into another group,they both also&re genetically closer.

     

/

返回文章
返回