红曲霉SAGE文库中双标签的制备研究

Preparation of ditags in constructing SAGE library of Monasccus Ruber

    摘要
  • 摘要: 研究探讨了红曲霉SAGE文库构建过程中总RNA提取、mRNA分离纯化、cDNA合成以及双标签(Ditags)制备方法，并通过PCR条件的优化获得双标签PCR的最佳模板稀释浓度及扩增循环数。

     

    Abstract: In this paper，the methods of total RNA and mRNA extraction，cDNA synthesis and ditags preparation are discussed in constructing SAGE library of Monasccus Ruher. The optima1 concentration of template and the number of PCR cycles are obtained by optimizing the PCR conditions．

     

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