Abstract: With the related analysis of elevation and climatic ecology quota of tobacco leaves and the analysis of chemical composition of tobacco leaves and smoke qualities appraisal in 7 counties (city)and 71 different elevation climatic ecology points for 3 years in Dalou Mountainous Region of the Northern of Guizhou，we can conclude that a very notable level of linear relation between the climatic ecology quota of tobacco leaves quality and elevation has rea ched and all climatic ecology quota mainly show the influence of elevation to tobacco leaves quality．The nicotine and total nitrogen contents reduce along with the elevation going up，and the quality is better in the districts of elevation 900～1100m ．The appearance quality，the inherent character，and the taste of tobacco lea ves decrease along with the elevation going up， and there is a slightly difference in the decrea sing range of different species in the same year and the same specie in different years．Adopting the corresponding technique measures just can acquire the high-quality tobacco leaves in the districts above the elevation 1300m ．