高山红景天毛状根培养的研究

Research of the hairy-root of Rhodiola sachalinensis A．Bor．

摘要: 建立了高山红景天毛状根培养系统，研究表明红景天子叶、子叶节能被A4、R1601、ATCC15834感染产生毛状根，PCR检测在500bp处有rolC基因存在。以1／2MS₀ 液体培养基、散射光、温度为20℃左右、pH 值4．5～4．8间毛状根生长为佳。对C源要求不高，N 源NH₄⁺ ：N0₃^- 为1：1时毛状根生长较好且红景天甙含量增加。

Abstract: The hairy-root culture system of Rhodiola sachalinensis A ．Bor was established in this paper．The results show that the hairy-root can be arisen at cotyledon and cotyledonary node infected by A4，R1601，ATCC15834 agrobacterium Ri-plasmid．There is about 500bp-rolC gene detected from the hairy-root culture by PCR amplifying． The hairy-root grows well at 1／2MS_o liquid medium，scatter light，20℃ and pH 4．5～4．8．In the condition of the lower nitrogen substance．such as NH₄⁺：N0₃^- equal to 1：1，the hairy-root grows well and the content of salidrls-ide has been increased．