Abstract: Ecological safety for agriculture sustainable development means that resources and environment on which the agriculture development depends are balanced and agro-ecosystem is health．There are many characteristics on ecological safety such as strategy，integration，region，gradation，dynamics and period．The paper points out four basic rules and a indicator system on ecological safety assessment．Taking a case study of Jiaxing City，Zhejiang Province as an example，the paper discusses the method and process of evaluation of ecological safety on agricultural sustainable development．