UV-B辐射增强对水稻多胺含量及其相关酶活性的影响

Effect of enhanced UV-B radiation on polyamine contents and the aetivity of related enzymines in rice (Oryza sativa L.)

  • 摘要: 试验研究表明,处理前期(7~14d)UV-B辐射增强使“汕优63”水稻精氨酸脱羧酶(ADC)、鸟氨酸脱羧酶(ODC)和S-腺苷蛋氨酸脱羧酶(SAMDC)活性分别增加165.74%、104.60%和89.6O%,“南川”(NC)这3种酶活性分别增加59.91%、41.3O%和23.68%,新品系“IR65600.85”在UV-B辐射胁迫下多胺脱羧酶活性表现与前2品种略不同,即ADC和0DC 活性分别提高115.93%、14.45%,而SAMDC 活性下降33.O1%。处理后期(21~28d)随UV-B辐射累积量的增加,这3种酶活性均有所下降,其中ODC和SAMDC活性降幅大于ADC,其中“汕优63”ADC和ODC活性分别比对照增加89.72% 、3.71%,“南川”则分别增加73.95%、27.38%,“IR56500.86”ADC活性增加94.41%,ODC活性却下降13.57%。处理后期(21~28d)3类水稻“汕优63”、“南川”和“IR65600.85”SAMDC分别下降4O.O6%、19.2O%和38.21%。多胺氧化酶(PAO)活性变化趋势则相反,即处理前期(7~14d)呈不同程度下降趋势,处理后期(21~28d)则呈极显著上升趋势,其结果引起多胺(PA)含量特别是腐胺(Put)含量明显上升。水稻对UV-B辐射增强的反应具有基因型差异。

     

    Abstract: Experimental results show that when the plants are exposed to enhanced UV-B radiation for 7~14 days.the activities of agrinine decarboxylase (ADC),ornithine decarboxylase(ODC)and s-adenosylmethionine decarboxylase(SAMDC)in leaves are increased by 165.74%,104.60%and 89.60%in“Shan You63”(Sy63),59.91%,41.30%and 23.68% in“Nancheum”(NC)and 115.93%,14.45%and-33.01%in IR65600.85,respectively.However,the three enzymic activities turn to decrease with the treatment time increasing(21~28d)and the decreased extents of ODC and SAMDC are much higher than that of ADC.The activities of ADC and ODC are increased by 89.72% and 3.71% in Sy63 and 73.95%and 27.38% in NC exposedto UV-B radiationfor 21~28 days.The activity of ADC is alsoincreased by 94.41% but ODC activity is decreased by 13.57% in the leaves of IR65600.85 compared with the controls.As far as SAM DC is concerned,the enzym ic activities in Sy63,NC and IR65600.85 are reduced by 40.06%,19.20% and 38.21%respectively when they are exposed to enhanced UV-B radiation for 21-28 days.The reverse is true in the case of polyamine oxidase(PAO),indicating its lower activity in earlier treatment courses(7~14d),but much higher activity in late courses(21~28d)compared with the controls.The in turn result in the increased contents of polyamine(PA),espedally putrescine(Put)and the results of the experiment have shown their differences in diverse rice genotypes.

     

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