Abstract: The allozyme patterns of AAT ，EST ，SOD，MDH，LDH ，and ME in heart，muscle，branchia，eye，kidney，liver，intestine，spleen and stomach of paralichthys olivaceus(T．et S．)were studied by using the assay of vertical slab polyacrylamide gel electrophoresis．The results indicate that four allozymes (EST，SOD，MDH，and ME)show the same expressions of allozyme in different tissues，and two allozymes (AAT，LDH)show the different expressions of allozyme in different tisues ，which may be controlled by diferent gene loci or different alleles in the same gene locus，are closely related to the tisues-specific biochemical metabolism．