应用多效唑常温保存甘薯试管种质的研究

A study of paclobutrazol(PP₃₃₃)on conservation of sweet potato germplasm in vitro at normal temperature

摘要: 以3．6～36．0umol／L浓度的多效唑处理“徐薯18”试管苗，可降低其生长强度，提高叶绿素含量和根冠比。在“MS+IBA 0．984umol／L”培养基中添加0．216～28．8umol／L浓度的多效唑，可使“徐薯18”试管苗在常温下保存1年以上。外源赤霉素能消除甘薯试管苗的多效唑后效应，0．289～0．434umol／L浓度的赤霉素可使保存后的甘薯试管苗生长量恢复到正常水平。

Abstract: Treated“Xushu 18”plantlets in vitro with PP₃₃₃ in each concentration between 3．6～36．0umol／L，the growth potential is decrea sed．but the content of chlorophyll and root-shoot ratio are increased．“Xushu 18” plantlets in vitro on the“MS+IBA0．984umol／L”medium can be kept for above one year by adding PP₃₃₃” in each concentration between 0．216～28．8umol／L．If exogenous GA₃ is added in“MS+IBAO．984umol／L”medium．the growth rate of sweet potato plantlets in vitro will return to normal leve1．The best concentration of GA₃ is 0．289～0．434umol／L．