Abstract: In this paper the isolation and purification of Striga kermontkica germination stimulant from Menispermum daurlcum DC．root culture filtrate are reported．Purification and isolation were undertaken by absorption of the stimulants onto XAD-4,solvent partitioning into ethyl acetate，and further separation on Sephadex LH20，C18 Sep-Pak Cartidge；preparative and analytical HPLC columns．Each step involved in the isolation procedure was followed by a bioassay using conditioned Striga hermonthica seeds．Three stimulants were detectcd in the filtrate． on HPLC the major stimulant showed similar chromatographic behavior and CO-eluted with authentic strigo1． Furthermore,its UV and Mass spectra were similar with those reported for strigol and strigol-like molecules． In this study，albeit no novel Striga germination stimulant was isolated from M ．dauricum root culture，the investigation represents the first report on isolation of strigol or strigol-like molecule from tisue culture．Moreover，it provides unequivocal evidence that strigol and strigol-like moleculas are of plant origin．