快速检测抗除草剂拿捕净基因流的方法初探

Probing into the method of prompt test of herbicide (Sethoxydim)-resistant gene flow

摘要: 实验探讨了快速检测抗除草剂拿捕净(Sethoxydim)基因流的方法，该方法核心是用双氧水和赤霉素(GA₃)打破青狗尾草种子的休眠，让其在含适宜剂量的除草剂基质上发芽，在暗处生长4d后仅通过测量种子发芽后的幼芽生长、形态差异即鉴定出抗、感除草剂种子，从而达到快速检测的目的。

Abstract: A method of testing herbicide(Sethoxydim )-resistant gene flow has been probed in this experiment The main point of the method includes three parts，break dormancy of green foxtail seeds with H₂O₂ and GA₃，weed seeds germinated on the medium containing aptitude dose of Sethoxydlm ，measure the young seedling growth after 4 days of germination．