西藏卓巴百合组织培养技术研究

Tissue culture of Lilium wardlii

摘要: 对西藏野生卓巴百合(Lilium wardlii stapf)进行组织培养结果表明，选用球茎内层、基部的鳞片作为外殖体，转绿率最高；在参试的培养基中MS+BA0．5mg／kg+NAA0．5～1．0mg／kg的培养基对诱导愈伤组织(EC)和不定芽的效果较好，MS+IBA1．0mg／kg对促进小鳞茎生根效果最佳；茎盘切块在MS+BA0．2～0．5mg／kg+NAA0．5～1 0mg／kg的培养基上能正常转绿，形成愈伤组织和不定芽；活性碳有助于小鳞茎生根；组培苗移栽到松锯末中其成活率可达到95％。

Abstract: Tissue culture of Lilium wardlii shows that the most perfect method of inducing EC(callus)and uncertain budlets belongs the medium of MS and BA 0．5mg／kg and NAA0．5～1．0mg／kg：the best medium inducing roots from littie bulbs is the medium of MS and IBA1．0mg／kg；the sterm cuts can be green and induce EC and uncertain budlets in the medium of MS and BA0．2～0．5mg／kg and NAA0．5～1．0mg／kg；the active carbon can help little bulbs striking roots when the tissue culture plants are moved to raise in the pine sawdust and their survival rate is 95 percent．