Abstract: This study deals with the integrated effects of nitrogen fertilizer on nitrate nitrogen accumulation in soil profile, yield and absorbed nitrogen of winter wheat in Huantai high-yielding wheat field system,Shandong Province.The results shown that under the condition of 150kg/hm² of nitrogen fetilizer,nitrate nitrogen amount in 0～90cm soil layer after cutting wheat in June changed a little,as compared with that prior to sowing wheat.It was also shown that the nitrate nitrogen amount in 0～90cm layer with routine rate of nitrogen fertilizer 300kg/hm² increased markedly,especially that in 60～90cm soil layer was raised from 10.3kg/hm² to 50.2kg/hm²,which might result in the pollution of shallow ground water.Under the condition of field experiment,150kg/hm² was recommended to be the rational nitrogen application rate,which could integrated yield and ecological profits.