幼虫密度对小菜蛾生长发育和繁殖的影响
Effects of larval density on growth, development and reproduction of diamondback moth (DBM), Plutella xylostella
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摘要: 为明确小菜蛾幼虫密度对其种群增长的影响, 在室内条件下温度(22±1) ℃, RH 70%~80%, 光周期 12L∶12D对不同幼虫密度1头·皿-1、10头·皿-1、20头·皿-1、30头·皿-1、40头·皿-1(直径为9 cm)饲养的小菜蛾生长发育及繁殖进行了研究。结果表明, 幼虫密度对小菜蛾幼虫、蛹的发育历期和存活率、蛹重及成虫生殖等有显著影响。幼虫和蛹历期均以10头·皿-1处理最短, 且随幼虫密度增加而显著延长。幼虫至蛹的存活率以10头·皿-1、20头·皿-1处理较高, 显著高于1头·皿-1和40头·皿-1处理。蛹重以1头·皿-1处理最重, 并随幼虫密度的增加而显著变轻。不同幼虫密度下羽化的成虫畸形率差异显著, 以10头·皿-1处理最低, 其余随幼虫密度的增加而升高。成虫产卵量以1头·皿-1处理最高, 随着幼虫密度增加, 产卵量逐渐下降。雌、雄蛾寿命分别以40头·皿-1、10头·皿-1处理最长, 密度过高时或过低雌雄蛾寿命显著缩短。生命表分析表明, 幼虫密度对小菜蛾种群增长指数影响显著, 以10头·皿-1处理种群增长指数最高, 幼虫密度过高或过低, 种群增长指数下降。因此, 幼虫密度是影响小菜蛾种群增长的重要因子之一。Abstract: This study investigated the effects of larval density on the population dynamics of diamondback moth (Plutella xylostella). It analyzed the growth, development and reproduction of P. xylostella of five larval densities (1 larvae·dish-1, 10 larvae·dish-1, 20 larvae·dish-1, 30 larvae·dish-1 and 40 larvae·dish-1) under laboratory conditions of (22±1) ℃, 70%~80% RH and 12L∶12D photoperiod. The results showed that immature duration, survival rate, pupal weight and adult reproduction of P. xylostella were significantly affected by larval density. The time of development of larva and pupa was longest at the density of 10 larvae per dish and significantly prolonged with increasing larval density. Survival rate from larva to pupa was highest at the density of 10 and 20 larvae per dish, which was significantly higher than those at the density of 1 and 40 larvae per dish. Pupal weight was heaviest at the density of one larvae per dish, which significantly dropped with increasing larval density. Abnormalities of moth adults were significantly different at different larval densities. Abnormality was lowest at the density of 10 larvae per dish, which significantly increased with increasing larval density. Total fecundity per female was highest at the density of one larva per dish, which also gradually declined with increasing larval density. Female and male longevities were highest at the densities of 40 and 10 larvae per dish respectively. It significantly shortened when the density was higher or lower. Life table analysis suggested that larval density significantly affected population growth index. It was highest at the density of 10 larvae per dish and dropped for both higher and lower densities. It was therefore concluded that larval density significantly influenced population growth of P. xylostella.