种植密度和方式对夏玉米叶绿体D1蛋白和PSII功能的影响

Effects of planting density and mode on summer maize chloroplastD1 protein and photo-system II

  • 摘要: 在豫北高产灌溉区生产条件下, 以"浚单20"、"郑单958"玉米为试验材料, 研究不同种植密度和种植方式下两种夏玉米生理指标和产量的变化。结果表明: 密度在6.75万~9.00万株·hm-2范围内, 随着种植密度的增加, 玉米穗位叶D1蛋白含量、可变荧光与最大荧光比(Fv/Fm)、光化学淬灭系数(qP)、光合速率(Pn)、SOD活性和单株产量降低, 非光化学淬灭系数(NPQ)、POD活性、MDA含量、秃尖长增加, 单位面积产量呈先增加后降低的趋势。密度≥8.25万株·hm-2时, Fv/Fm、qPPn、叶片SOD活性急剧降低, 密度9.00万株·hm-2比8.25万株·hm-2降低1.2%~21.7%; NPQ、MDA含量、POD活性急剧升高, 密度9.00万株·hm-2比8.25万株·hm-2升高3.2%~15.0%。Fv/Fm、qPPn、SOD活性宽窄行种植模式高于等行距模式, NPQ、MDA含量、POD活性宽窄行种植模式低于等行距模式。密度≥7.50万株·hm-2后, "郑单958"的Fv/Fm、qPPn、SOD活性高于"浚单20", NPQ、MDA含量、POD活性低于"浚单20"。品种、种植方式和密度三因素对D1蛋白含量, Fv/Fm、qPPn、NPQ, SOD、POD活性, MDA含量及产量影响差异显著, 且三因素互作的影响也差异显著。两种玉米的最适种植方式为密度不高于8.25万株·hm-2且宽窄行种植。

     

    Abstract: The effects of different planting densities and modes on grain yield and physiological parameters of two summer maize varieties ("Zhengdan 958" and "Xundan 20") were studied under high yield conditions in North Henan Province. With 67 500~90 000 plants per hm-2, D1 protein content, optimal photochemical efficiency of PSII (Fv/Fm), photochemical quenching coefficient (qP), photosynthetic rate (Pn) and activities of foliar superoxide dismutase (SOD) decreased. However, peroxidase (POD) activity, non-photochemical quenching (NPQ) and malondialdehyde (MDA) content increased. At the same time, bald needle length increased and ear weight decreased. Yield increased to a peak then decreased with increasing plant density. When plant density exceeded 82 500 plants per hm-2, Fv/Fm, qP, Pn and SOD activity decreased dramatically. These parameters were lower at densities of 90 000 plants per hm-2 than at 82 500 plants per hm-2; dropping by an average from 1.2%~21.7%. NPQ, POD activity, and MDA content increased with increasing plant density. These parameters were also higher at densities of 90 000 plants per hm-2 than at 82 500 plants per hm-2, increasing by an average 3.2%~15.0%. Fv/Fm, qP, Pn and SOD activity in wide-narrow row planting mode were higher than in equidistant row planting mode, while NPQ, POD activity and MDA content exhibited the polar opposite. When the density exceeded 75 000 plants per hm-2, Fv/Fm, qP, Pn and SOD activity in "Zhengdan 958" were higher than those in "Xundan 20", but NPQ, POD activity and MDA content exhibited the polar opposite. D1 protein content, Fv/Fm, qP, NPQ, Pn, activities of SOD and POD, MDA content, bald needle length, ear weight and yield were significantly influenced by variety, plant density and planting mode. The interactions of the three factors were distinctively significant. Yield was highest at a density of 82 500 plants per hm-2 in the wide-narrow row planting mode for the two summer maize varieties.

     

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