Abstract: In this study, we determined the enhancement effects of Trichoderma aureoviride 1010 (T1010) on chilly stress-resistance of tomato in solar-greenhouse conditions via soil application and 3 days of inferior chilly-stress (16 ± 2 °C in the day and 8 ± 2 °C at night ). Results show significant differences in the indicators for inferior chilly stress-resistance of functional tomato leaves and roots. SOD, CAT, POD and PPO activities of functional tomato leaves treated with T1010 increase by 266.33%, 16.85%, 20.89% and 632.89% respectively over the control. Carotenoid, ABA, proline, glucose and starch contents also increase by 50.53%, 119.40%, 55.38%, 128.57% and 23.40% respectively. For tomato roots treated with T1010, SOD, CAT, POD and PPO activities increase by 274.10%, 69.06%, 8.26% and 164.14% respectively. The contents of proline, glucose, and starch also increase by 49.81%, 66.00% and 86.76% respectively. In addition to decreasing soluble sugar content, the indicators for functional tomato leaves treated with 1/2T1010 increase at varying degrees. However, the indicators for tomato roots treated with 1/2T1010 decrease at varying degrees. ANOVA analysis shows significant difference in indicators of inferior chilly stress-resistance among CK, T1010 and 1/2T1010 treatments. T1010 positively influences chilly stress-resistance of functional tomato leaves and roots in solar-greenhouse conditions with reasonable soil organic quality.