Abstract: Root zone soil nutrient change in different vineyards respectively replanted 3 times with 30 years cultivation and not replanted with 3 years cultivation of grapes was studied. Growth of replanted grapes in root zone soil from different vineyards was also investigated through potted experiment. The results indicate that soil organic matter and macro-elements (N, P, K and available Ca and Mg) increase with increasing grape planting years. However, the dynamics of trace elements is a lot more complicated. Available Fe and Mn are deficit in replanted vineyard soils, while available Cu, Zn increase. Zn/Mn, Zn/Fe, N/Fe, P/Fe, Zn/K are imbalance in root zones, of which Zn/Mn and Zn/Fe are the worst state of balance. Compared to newly planted vineyards, average plant height, stem diameter, shoot and root fresh weight, root activity and leaf chlorophyll content of replanted vineyards separately drop by 39.80%, 5.82%, 47.97%, 30.17%, 21.22% and 30.36%, along with remarkable replant diseases. Available Fe deficiency and Zn/Mn and Zn/Fe imbalances are the probable factors associated with replant obstacles of grape.