Effect of combined application of Chinese milk vetch and nitrogen fertilizer on nitrogen uptake, utilization and dry matter accumulation in early rice
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摘要: 为综合评价紫云英与氮肥配施对早稻干物质生产及氮素吸收利用的影响，筛选紫云英等量翻压条件下，较适宜的施氮水平，以冬闲常规施氮[150 kg（N）·hm-2]处理为对照，在翻压紫云英22 500 kg·hm-2条件下，设置90 kg（N）·hm-2、120 kg（N）·hm-2、150 kg（N）·hm-2和180 kg（N）·hm-2 4个施氮水平，研究紫云英和施氮量对早稻干物质生产及氮素吸收利用的影响。结果表明：紫云英与氮肥配施各处理的干物质积累量均高于对照，其中紫云英配施氮肥90 kg（N）·hm-2和120 kg（N）·hm-2的干物质积累量最多，分别达9.65 t·hm-2和9.97 t·hm-2，比对照分别增加11.18%和14.86%。各处理在水稻播种-分蘖期及抽穗-灌浆期干物质积累量较大，占成熟期干物质量的19.26%~24.77%和45.23%~52.75%，这两个生育阶段是干物质主要积累时期。紫云英与氮肥配施各处理的氮素积累量均高于对照，增幅为6.95%~18.68%。氮素干物质生产效率和氮收获指数均以紫云英配施90 kg（N）·hm-2处理最高，比其他处理分别增加3.94%~14.08%和6.65%~14.90%。紫云英配施氮肥有利于提高早稻的干物质积累量和氮素利用率，其中以紫云英配施氮肥90 kg（N）·hm-2和120 kg（N）·hm-2效果较优，可实现减氮增效目的，是较理想的施肥模式。Abstract: This study evaluated the effect of combined application of Chinese milk vetch (Astragalus sinicus L.) and different levels of nitrogen (N) fertilizer on dry matter accumulation and N use efficiency of early rice in order to determinate the suitable N level under application of Chinese milk vetch. Field treatments were set with four N application levels (90 kg·hm-2, 120 kg·hm-2, 150 kg·hm-2 and 180 kg·hm-2) under 22 500 kg·hm-2 Chinese milk vetch using the winter fallow field with general N fertilizer level (150 kg·hm-2) as the control. In the experiment, Chinese milk vetch was planted in winter and incorporated into soil in spring before early rice transplant, while N fertilizer was applied as base fertilizer, tillering fertilizer and heading fertilizer with 5:3:2 rate. The rice plants were sampled at tillering, booting, heading, filling and maturity stages of the early rice. Dry matter weight and N content were assayed, and the relative indexes were calculated. The results showed that all N application treatments had higher dry matter accumulation than the control. Specifically, the treatments with Chinese milk vetch combined with 90 kg (N)·hm-2 and 120 kg (N)·hm-2 increased dry matter (9.65 t·hm-2 and 9.97 t·hm-2) respectively by 11.18% and 14.86% compared to the control. Dry matter accumulation increased with duration of growth and peaked twice, one at sowing-tillering stage (19.26%-24.77%) and the other at heading-filling stage (45.23%-52.75%). The combined application of Chinese milk vetch and N fertilizer supported N integration that was beneficial to rice growth. The amounts of N accumulation of early rice treated with Chinese milk vetch and N fertilizer were higher than the control, with increases of 6.95%-18.68%. Among the treatments, that of Chinese milk vetch combined with 90 kg (N)·hm-2 had the highest production efficiency of dry matter and N harvest index, with respective increases of 3.94%-14.08% and 6.65%-14.90% over other treatments. The results indicated that combined application of Chinese milk vetch with 90 kg (N)·hm-2 or 120 kg (N)·hm-2 was satisfactory because they reduced N fertilizer and increased N use efficiency at the same time, which was the ideal fertilization model for the study area.
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Keywords:
- Chinese milk vetch /
- Nitrogen fertilizer /
- Early rice /
- Dry matter /
- Nitrogen uptake
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紫云英 (Astragalus sinicus L.) 是我国南方稻区传统的冬绿肥, 翻压后能够增加土壤养分、改善土壤理化性状、提高植株氮磷钾含量、减少化肥用量[1-3]。近几十年来, 随着我国农业集约化程度的加深, 化肥因具有便捷、增效快等优点成为粮食增产的主要依赖对象, 这使我国普遍存在忽视绿肥施用、过度依赖化肥的现象, 化肥特别是氮肥用量过大, 目前我国水稻平均氮肥施用量为180 kg∙hm-2, 较世界平均水平增加75%, 有些地区甚至超过300 kg∙hm-2[4-5]。而过量施用氮肥会降低氮素利用效率[6], 造成作物贪青晚熟, 并引发一系列环境问题[7]。因此, 利用冬季空闲茬口种植紫云英, 并进行合理的养分管理, 在维持水稻产量高产的同时提高氮肥利用率, 对于实现水稻优质、高产、高效, 减少肥料损失具有十分重要的意义。国内外关于绿肥与化肥配施对水稻增产效果的研究已有不少报道, 多数研究表明, 利用紫云英根瘤菌固氮, 并配施适量化肥, 改善了土壤肥力, 其增产效果较好[8-9]。谢志坚等[10]通过研究翻压等量紫云英配施不同量化肥的土壤养分有效性表明, 紫云英配施60%~80%常规施肥量的化肥 (常规施肥为: N 150 kg∙hm-2、P2O575 kg∙hm-2、K2O 120 kg∙hm-2), 土壤中的碱解氮和速效钾能够增加10%~59%。王建红等[11]研究了紫云英还田配施化肥对单季晚稻养分利用和产量的影响, 结果表明紫云英鲜草翻压配施化肥处理的稻谷产量比不施肥处理提高8.5%~17.4%。侯红乾等[12]连续25年在江西省双季稻区进行田间定位试验, 研究表明, 有机无机肥配施条件下早晚稻平均产量比单施化肥增产3.9%~7.8%。徐昌旭等[13]研究结果表明, 早稻减少化肥用量20%(常规施肥为: N 150 kg∙hm-2、P2O575 kg∙hm-2、K2O 120 kg∙hm-2) 能有效促进水稻植株对氮、磷、钾养分的吸收与积累。国内外学者关于作物合理施肥研究很多, 但对于通过绿肥种植翻压减少化肥施用的水稻环境友好型栽培条件下水稻氮肥适宜用量的研究报道较少。而很多绿肥还田的研究多以紫云英还田与化肥配施 (或有机无机肥配施) 为重点, 对紫云英配施氮肥的研究不够系统充分。本研究利用冬季空闲茬口种植紫云英, 通过比较紫云英还田配施不同氮肥施用量下水稻干物质生产特性及氮素吸收利用的情况, 对冬种紫云英条件下稻田的合理施氮量进行探究, 从而为水稻可持续生产和水稻保护性耕作提供理论依据。
1. 材料与方法
1.1 试验区概况
试验于2014年10月至2015年7月在江西农业大学科技园水稻实验田 (28°46′N, 115°55′E) 进行。试验区为亚热带季风性湿润气候, 年均太阳总辐射量4.79×1013 J·hm-2, 年均日照时数1 852 h, 年日均温≥0 ℃积温达6 450 ℃, 年平均气温17.1~17.8 ℃, 年降水量1 624 mm。供试土壤为发育于第四纪的红黏土, 为亚热带典型红壤分布区。紫云英种植前试验耕层土壤pH 5.59, 有机质29.48 g∙kg-1, 全氮2.17 g∙kg-1, 碱解氮38.69 mg∙kg-1, 有效磷12.22 mg∙kg-1, 速效钾30.31 mg∙kg-1。
1.2 试验设计
采取单因素随机区组设计, 以冬闲常规施氮处理[150 kg (N)·hm-2]为对照 (处理A), 在等量翻压紫云英鲜草22 500 kg∙hm-2(干草养分含量:全氮26.7 g∙kg-1, 全磷2.1 g∙kg-1, 全钾20.1 g∙kg-1) 条件下, 施氮量设90 kg (N)∙hm-2(处理B)、120 kg (N)∙hm-2(处理C)、150 kg (N)∙hm-2(处理D) 和180 kg (N)∙hm-2(处理E)4个水平, 共5个处理, 所有处理3次重复, 15个小区, 小区面积为16.5 m2(5.5 m×3 m), 小区之间用水泥埂隔开, 以防止水肥串流。供试紫云英品种为‘余江大叶籽’, 2014年10月4日播种, 2015年4月3日盛花期翻压。早稻品种为‘金优458’, 于2015年3月27日播种, 4月29日移栽, 7月28日收割。每兜3苗, 每小区325兜。各处理氮肥用尿素 (含N 46%), 磷肥用钙镁磷肥 (含P2O512%), 钾肥用氯化钾 (含K2O 60%)。磷肥、钾肥各小区施用量相同, 磷肥 (P2O5)50 kg∙hm-2, 钾肥 (K2O)120 kg∙hm-2, 全部作基肥; 氮肥按基肥︰分蘖肥︰穗肥=5︰3︰2施用。分蘖肥在水稻移栽后5~7 d时施用, 穗肥在主茎幼穗长1~2 cm时施用。田间管理措施同一般大田栽培。
1.3 测定指标及计算方法
1.3.1 干物质测定
于早稻分蘖期 (2015年6月1日)、孕穗期 (6月14日)、抽穗期 (6月25日)、灌浆期 (7月6日) 和成熟期 (7月25日) 按每小区茎蘖数的平均数取代表性植株5穴 (小区边行不取), 分成叶片、茎鞘和穗 (抽穗后) 等部分装袋, 105 ℃下杀青30 min, 80 ℃下烘干至恒重后称重。干物质转运相关指标计算方法[14]分别为:
$$ 茎叶干物质输出量=抽穗期茎叶干物重-成熟期茎叶干物重 $$ (1) $$ \text{茎叶干物质输出率}=\text{茎叶干物质输出量}/\text{抽穗期茎叶干物重}\times 100{\text{% }} $$ (2) $$ 表观转变率=茎叶干物质输出量/抽穗至成熟期穗部干物质积累量\times 100{\text{% }} $$ (3) 1.3.2 植株氮素测定
每时期植株干物质积累测定完成后粉碎混匀, 采用H2SO4-H2O2消化, 以半微量开氏定氮法测定植株各器官全氮含量。相关指标计算方法[15]分别为:
$$ 氮素积累量=该时期地上部干物重\times 含氮量 $$ (4) $$ \text{氮素干物质生产效率(NDMPE)=}单位面积植株干物质积累量/植株氮积累量 $$ (5) $$ \text{氮收获指数(NHI)=}籽粒氮素积累量/植株氮素积累量 $$ (6) 1.4 数据处理
本研究所有数据的基本统计采用Microsoft Excel 2010, 采用SPSS 17.0软件进行统计分析。
2. 结果与分析
2.1 紫云英与氮肥配施对早稻干物质积累的影响
2.1.1 主要生育期群体干物重
由表 1可知, 不同施肥条件下早稻主要生育期地上部群体干物重因生育期不同而有所差异。分蘖期各处理间差异不显著 (P > 0.05)。从孕穗期开始, 各处理的差异逐渐显现。除分蘖期以外, 其余生育期处理C的干物质积累量均达最大, 且在孕穗期与其他处理间差异显著 (P < 0.05)。在抽穗期, 处理B、C、D的干物质积累量显著高于A、E处理 (P < 0.05)。在灌浆期, 处理B、C干物质累积量显著高于处理A (P < 0.05), 增幅分别为16.77%和17.02%。在成熟期, 处理B、C、D干物质累积量显著高于处理A (P < 0.05), 且处理C与处理A、E差异显著 (P < 0.05)。
表 1 各处理对早稻主要生育期群体干物重的影响Table 1. Effects of different treatments on dry matter weight of population atin main growth periods stages of early rice t·hm-2t·hm2 处理
Treatment分蘖期
Tillering stage孕穗期
Booting stage抽穗期
Heading stage灌浆期
Filling stage成熟期
MaturityA 2.15±0.12a 2.62±0.05c 3.78±0.09b 8.05±0.24b 8.68±0.14c B 1.95±0.06a 3.06±0.06b 4.45±0.04a 9.40±0.15a 9.65±0.27ab C 1.92±0.14a 3.58±0.17a 4.66±0.13a 9.42±0.42a 9.97±0.24a D 2.09±0.07a 3.04±0.16b 4.54±0.12a 8.81±0.46ab 9.44±0.29ab E 2.16±0.09a 2.70±0.04bc 3.78±0.02b 8.58±0.17ab 9.10±0.06bc A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level. 2.1.2 主要生育阶段干物质积累量和比例
从早稻主要生育阶段干物质积累量和比例来看 (表 2), 水稻播种—分蘖期及抽穗—灌浆期是干物质主要积累时期, 水稻在这两个生育阶段干物质积累量较大, 各处理在这两个生育阶段干物质积累量占成熟期干物重的19.26%~24.77%和45.23%~52.75%, 但均未达显著性差异 (P > 0.05)。在分蘖—孕穗期处理C的干物质积累量最大, 且显著高于处理A、D、E (P < 0.05);在孕穗—抽穗期, 处理D的干物质积累量最大, 显著高于处理C、E (P < 0.05), 增幅均为38.89%;而灌浆—成熟期各处理间干物质积累量差异不显著 (P > 0.05)。
表 2 各处理早稻主要生育阶段干物质积累量和比例Table 2. Dry matter accumulation and its ratio in main growth stages of early rice under different treatments处理
Treatment播种—分蘖期
Sowing-tillering stage分蘖—孕穗期
Tillering-booting stage孕穗—抽穗期
Booting-heading stage抽穗—灌浆期
Heading-filling stage灌浆—成熟期
Filling-maturity stage积累量
DMA
(t·hm-2)比例
RTDM
(%)积累量
DMA
(t·hm-2)比例
RTDM
(%)积累量
DMA
(t·hm-2)比例
RTDM
(%)积累量
DMA
(t·hm-2)比例
RTDM
(%)积累量
DMA
(t·hm-2)比例
RTDM
(%)A 2.15±0.12a 24.77 0.47±0.11b 5.41 1.16±0.11ab 13.36 4.27±0.30a 49.19 0.63±0.27a 7.27 B 1.95±0.06a 20.21 1.11±0.12ab 11.50 1.39±0.06ab 14.40 4.95±0.13a 51.30 0.25±0.40a 2.59 C 1.92±0.14a 19.26 1.66±0.28a 16.65 1.08±0.17b 10.83 4.77±0.44a 47.84 0.55±0.27a 5.52 D 2.09±0.07a 22.15 0.95±0.09b 10.06 1.50±0.04a 15.89 4.27±0.34a 45.23 0.63±0.18a 6.67 E 2.16±0.09a 23.74 0.54±0.13b 5.93 1.08±0.03b 11.87 4.80±0.19a 52.75 0.52±0.14a 5.71 A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。DMA:生育阶段干物质积累量; RTDM:该生育阶段干物质积累量占总积累量的比重。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. DMA: amount of dry matter accumulation in at the growth stage; RTDM: the ratio of dry matter accumulation to total accumulation in theis growth stage. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level. 2.1.3 干物质在茎鞘、叶片和穗的分配情况
通过分析茎鞘、叶片和穗分配情况 (表 3) 可知, 早稻干物质茎鞘比例在抽穗期达最大, 并随着生育进程的推进有不断降低的趋势。抽穗期处理C的茎鞘比例最大, 达46.50%, 与处理A、E差异显著 (P < 0.05), 分别增加7.83个百分点和4.75个百分点。叶片比例的变化趋势与茎鞘比例一致, 在抽穗期达最大, 各处理间差异显著 (P < 0.05), 其中处理A叶片比例最大, 为41.33%;成熟期处理D的叶片比例最大, 为14.00%, 与处理A、B、E差异显著 (P < 0.05)。穗比例随着生育进程的推进呈现出不断上升的趋势, 各处理均在成熟期达最大, 从抽穗期的18.50%~23.50%增加到成熟期的64.50%~68.67%。抽穗期处理D的穗比例最大, 并与处理C差异显著 (P < 0.05);成熟期处理B与处理A、C、D差异显著 (P < 0.05)。
表 3 各处理早稻中、后期干物质在茎鞘、叶片和穗的分配情况Table 3. Dry weight ratios of stem-sheath, leaf, panicle at middle and late stages of early rice under different treatments %% 处理
Treatment茎鞘比例Ratio of stem-sheath 叶片比例Ratio of leaf 穗比例Ratio of panicle 抽穗期
Heading stage灌浆期
Filling stage成熟期
Maturity stage抽穗期
Heading stage灌浆期
Filling stage成熟期
Maturity stage抽穗期
Heading stage灌浆期
Filling stage成熟期
Maturity stageA 38.67±0.33c 28.00±0.58a 22.00±0.58a 41.33±0.33a 18.00±0.00a 11.67±0.67bc 20.00±0.58ab 54.00±0.58a 66.33±0.33bc B 43.33±0.67ab 27.67±1.67a 20.67±1.20a 36.67±0.33c 21.66±2.67a 10.67±0.33c 20.00±1.00ab 50.67±4.33a 68.67±0.89a C 46.50±1.44a 26.25±1.25a 20.75±0.75a 35.00±0.41d 17.75±0.75a 13.25±0.48ab 18.50±1.71b 56.00±1.87a 66.00±0.71bc D 43.50±0.50ab 26.50±0.50a 21.50±0.50a 33.00±0.00e 19.00±0.00a 14.00±0.00a 23.50±0.50a 54.50±0.50a 64.50±0.50c E 41.75±0.75bc 28.50±0.87a 20.25±0.25a 38.25±0.63b 18.50±0.29a 12.00±0.41bc 20.00±1.29ab 53.00±1.00a 67.75±0.25ab A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level. 2.1.4 干物质的转运
从表 4可知, 处理B的早稻茎叶干物质输出量、输出率和表观转变率均达到最大, 处理C次之, 处理E达到最低, 处理D的3个指标高于处理A, 但未达到显著性差异 (P > 0.05)。说明在紫云英全量还田条件下, 减量施氮促进了早稻地上部分营养器官的干物质向籽粒中转移, 且相同施氮量下, 紫云英还田与否对干物质从营养器官向籽粒中转移效果并不明显, 而高量施氮反而抑制了干物质的转移。
表 4 各处理早稻干物质的转运Table 4. Dry matter transformation of early rice under different treatments处理
Treatment茎叶干物质输出量
Dry matter exportation from in stem-sheath and leaf (t·hm-2)茎叶干物质输出率
Output percent of dry matter fromin stem-sheath and leaf (%)表观转变率
Apparent conversion rate (%)A 0.11±0.01c 3.31±0.07c 2.20±0.06c B 0.54±0.01a 15.17±0.43a 9.41±0.08a C 0.41±0.02b 10.79±0.43b 7.17±0.10b D 0.12±0.01c 3.46±0.08c 2.39±0.12c E 0.09±0.01c 2.98±0.10c 1.66±0.07d A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+ 120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level. 2.2 紫云英与氮肥配施对早稻氮素含量的影响
2.2.1 主要生育期氮素吸收积累量
由图 1可知, 紫云英配施氮肥条件下早稻主要生育期氮素吸收积累量因生育期不同而有所差异, 且呈逐渐升高的趋势, 在成熟期达最大。分蘖期各处理间氮素吸收积累量差异不显著 (P > 0.05)。除抽穗期处理D的氮素积累量最大外, 孕穗期、灌浆期和成熟期都是处理C的氮素积累量达最大, 且均显著高于处理A, 其中灌浆期和成熟期亦显著高于处理B (P < 0.05)。
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图 1 各处理早稻主要生育阶段氮素累积量A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+ 180 kg (N)·hm-2。不同小写字母分别表示同一生育时期各处理间差异显著 (P < 0.05)。Figure 1. Nitrogen accumulations in at main growth periods stages of early rice under different treatmentsA: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Different lowercase letters mean significant differences at the same growth stage at 0.05 level.2.2.2 成熟期茎、叶、穗的氮素养分含量及吸收量
由表 5可知, 不同施肥处理成熟期早稻茎、叶、穗氮素养分含量存在差异, 且不同施肥处理对各养分器官的氮素养分吸收量存在较为明显的影响。从茎全氮含量看, 处理C、D、E均显著高于处理B (P < 0.05), 可能是紫云英还田条件下, 较高施氮量一定程度上促进了茎中氮素的累积, 也可能是由于处理B本身的施氮量较其他处理少; 从叶全氮含量看, 各处理间并无显著差异; 从穗全氮含量看, 也是处理E显著高于其他处理 (P < 0.05)。从茎氮素吸收量看, 处理C显著高于处理B (P < 0.05);从叶片氮素吸收量看, 处理D显著高于处理B (P < 0.05);从穗氮素吸收量看, 4种紫云英配施氮肥处理均显著高于处理A (P < 0.05)。
表 5 各处理早稻成熟期茎、叶、穗的氮素养分含量及吸收量Table 5. Nitrogen contents and nutrient uptakes of stem-sheath, leaf, panicle in at maturity stages of early rice under different treatments处理
Treatment全氮含量Total nitrogen content (%) 氮素养分吸收量Nitrogenutrient uptakes (kg·hm-2) 茎Stem-sheath 叶Leaf 穗Panicle 茎Stem-sheath 叶Leaf 穗Panicle A 1.14±0.13ab 2.08±0.15a 1.45±0.04bc 21.67±1.97ab 21.03±1.24ab 83.60±1.49b B 1.01±0.04b 1.83±0.15a 1.45±0.00bc 19.96±0.93b 19.16±1.70b 95.96±2.20a C 1.36±0.03a 1.81±0.07a 1.41±0.02c 33.48±5.64a 23.73±1.58ab 92.68±2.77a D 1.34±0.11a 1.87±0.02a 1.50±0.03b 27.52±3.63ab 24.29±0.58a 91.33±4.02a E 1.43±0.10a 2.03±0.06a 1.57±0.01a 26.61±1.99ab 21.92±0.73ab 96.80±1.05a A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level. 2.2.3 氮素养分吸收利用效率
由表 6可知, 不同施肥处理下早稻的氮素养分吸收利用效率存在差异。从氮素干物质生产效率 (NDMPE) 看, 氮素干物质生产效率表现出随施氮量的增加而下降的趋势。处理B的氮素干物质生产效率最高, 比其他处理增加3.94%~14.08%。氮收获指数 (NHI) 反映了氮素在植株营养器官与生殖器官间的分配情况, 结果显示不同施肥处理对早稻氮收获指数存在一定的影响。各处理氮收获指数为61.83%~71.04%, 其中处理B的氮收获指数显著高于其他处理 (P < 0.05), 处理E次之。因此, 在各处理中, 处理B的氮素干物质生产效率和氮收获指数较高, 分别为71.44 kg·kg-1和71.04%。
表 6 各处理早稻的氮素干物质生产效率 (NDMPE) 和氮收获指数 (NHI)Table 6. Nitrogen dry matter production efficiency (NDMPE) and nitrogen harvest index (NHI) of early rice under different treatments处理Treatment NDMPE (kg·kg-1) NHI (%) A 68.73±4.31a 66.19±1.08bc B 71.44±3.08a 71.04±1.45a C 66.52±2.87a 61.83±1.35c D 65.95±7.97a 63.80±1.43bc E 62.62±1.84a 66.61±1.08b A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+ 120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level. 3. 讨论与结论
研究表明, 有机无机肥配施有利于促进植株干物质积累[16]。徐昌旭等[13]研究结果显示, 翻压22 500 kg∙hm-2紫云英后, 与施用100%化肥 (N 150 kg∙hm-2、P2O575 kg∙hm-2、K2O 120 kg∙hm-2) 相比, 减少化肥用量可以促进干物质的积累; 减少化肥用量20%, 水稻干物质积累量平均增加18.8%, 而化肥用量减少到常规用量的40%~60%时, 干物质积累量并没有减少。杨长明等[17]研究结果表明:有机无机肥配施有利于水稻植株在灌浆期对干物质的积累。姜佰文等[18]研究结果显示, 在等量有机肥用量条件下, 配施适宜比例的无机肥能够提高总干物质积累量18.8%~26.9%。本研究结果同样表明, 与对照处理相比, 紫云英与氮肥配施各处理的早稻干物质积累量均在一定程度上有所提高, 其中以紫云英配施120 kg∙hm-2氮肥处理的干物质积累量最大。各处理在抽穗—灌浆期的干物质积累比例均最大, 而紫云英与氮肥配施处理在这一阶段的干物质积累量较冬闲处理平均增加10.01%。聂俊等[19]研究结果表明, 有机肥与化肥配施可以提高水稻分蘖期到成熟期的干物质积累, 而本研究结果显示紫云英与氮肥配施对干物质积累的优势仅从孕穗期开始, 在分蘖期冬闲常规施氮处理、紫云英配施150 kg∙hm-2氮肥和180 kg∙hm-2氮肥处理的干物质积累量均高于紫云英配施90 kg∙hm-2氮肥和120 kg∙hm-2氮肥处理, 这可能是因为紫云英翻压后由于前期气温低, 氮素释放慢, 并未完全转化成可被直接利用的形式, 氮素供应相对不足[20], 而氮肥作为速效肥料, 养分释放速度较快, 因此氮肥施用量大的处理在水稻生育前期长势较好, 而紫云英与氮肥配施处理在水稻生育中后期长势较好。从早稻干物质转运情况看, 随着施氮量的增加, 茎叶干物质输出量、输出率及表观转变率呈下降趋势, 这与朱冰[21]、赵田径等[22]的研究结果一致。
养分吸收是物质生产的基础。植物中的养分从土壤中获得, 杨馨逸等[23]认为在相同肥力下, 土壤氮素转化率随着施氮量的增加呈现先上升后下降的趋势, 只有施入适宜的氮量才能协调好土壤供氮和作物需氮之间的关系。因此, 氮肥施用不合理、养分供应不同步是氮素利用效率低的主要原因[24]。常用的氮肥是速效肥料, 冬闲处理在水稻生长前期供肥过旺、后期供肥不足, 而紫云英与化肥的养分释放速率不同, 翻压紫云英处理在水稻全生育期都有充足的养分供应。要文倩等[25]研究结果表明, 有机无机肥配施有利于提高水稻养分利用效率, 促进氮素吸收。商跃凤[26]研究表明有机无机氮肥混施氮肥利用率比单施化肥提高7%~18%。张小莉等[27]研究结果亦表明, 有机无机复混肥处理的氮素积累量、氮素利用效率均显著高于化肥处理。本试验中, 紫云英与氮肥配施增加了水稻的氮素吸收量, 与不施紫云英处理相比, 紫云英与氮肥配施处理的早稻氮素吸收量增加6.95%~18.68%, 其中紫云英配施120 kg∙hm-2氮肥处理的氮素吸收积累量最高。这与李贺[28]、孟琳[29]的研究结果类似。本试验中, 氮素干物质生产效率随着施氮量增加呈先上升再下降的趋势, 说明在一定范围内, 施氮量增加能促进作物对氮素的吸收利用, 但当氮肥施用量过量施用时, 会造成水稻对氮素的奢侈吸收[30], 从而降低氮素利用率, 这与前人[11]的研究结果一致。
本试验条件下, 与不施紫云英处理相比, 紫云英配施氮肥处理提高了水稻干物质积累量、氮素吸收积累量以及氮素利用率, 其中以紫云英配施纯氮90 kg∙hm-2和120 kg∙hm-2处理的效果较优, 明显地实现了减氮增效的目的, 是较理想的施肥模式。
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图 1 各处理早稻主要生育阶段氮素累积量
A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+ 180 kg (N)·hm-2。不同小写字母分别表示同一生育时期各处理间差异显著 (P < 0.05)。
Figure 1. Nitrogen accumulations in at main growth periods stages of early rice under different treatments
A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Different lowercase letters mean significant differences at the same growth stage at 0.05 level.
表 1 各处理对早稻主要生育期群体干物重的影响
Table 1 Effects of different treatments on dry matter weight of population atin main growth periods stages of early rice t·hm-2
t·hm2 处理
Treatment分蘖期
Tillering stage孕穗期
Booting stage抽穗期
Heading stage灌浆期
Filling stage成熟期
MaturityA 2.15±0.12a 2.62±0.05c 3.78±0.09b 8.05±0.24b 8.68±0.14c B 1.95±0.06a 3.06±0.06b 4.45±0.04a 9.40±0.15a 9.65±0.27ab C 1.92±0.14a 3.58±0.17a 4.66±0.13a 9.42±0.42a 9.97±0.24a D 2.09±0.07a 3.04±0.16b 4.54±0.12a 8.81±0.46ab 9.44±0.29ab E 2.16±0.09a 2.70±0.04bc 3.78±0.02b 8.58±0.17ab 9.10±0.06bc A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level. 
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表 2 各处理早稻主要生育阶段干物质积累量和比例
Table 2 Dry matter accumulation and its ratio in main growth stages of early rice under different treatments
处理
Treatment播种—分蘖期
Sowing-tillering stage分蘖—孕穗期
Tillering-booting stage孕穗—抽穗期
Booting-heading stage抽穗—灌浆期
Heading-filling stage灌浆—成熟期
Filling-maturity stage积累量
DMA
(t·hm-2)比例
RTDM
(%)积累量
DMA
(t·hm-2)比例
RTDM
(%)积累量
DMA
(t·hm-2)比例
RTDM
(%)积累量
DMA
(t·hm-2)比例
RTDM
(%)积累量
DMA
(t·hm-2)比例
RTDM
(%)A 2.15±0.12a 24.77 0.47±0.11b 5.41 1.16±0.11ab 13.36 4.27±0.30a 49.19 0.63±0.27a 7.27 B 1.95±0.06a 20.21 1.11±0.12ab 11.50 1.39±0.06ab 14.40 4.95±0.13a 51.30 0.25±0.40a 2.59 C 1.92±0.14a 19.26 1.66±0.28a 16.65 1.08±0.17b 10.83 4.77±0.44a 47.84 0.55±0.27a 5.52 D 2.09±0.07a 22.15 0.95±0.09b 10.06 1.50±0.04a 15.89 4.27±0.34a 45.23 0.63±0.18a 6.67 E 2.16±0.09a 23.74 0.54±0.13b 5.93 1.08±0.03b 11.87 4.80±0.19a 52.75 0.52±0.14a 5.71 A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。DMA:生育阶段干物质积累量; RTDM:该生育阶段干物质积累量占总积累量的比重。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. DMA: amount of dry matter accumulation in at the growth stage; RTDM: the ratio of dry matter accumulation to total accumulation in theis growth stage. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level.
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表 3 各处理早稻中、后期干物质在茎鞘、叶片和穗的分配情况
Table 3 Dry weight ratios of stem-sheath, leaf, panicle at middle and late stages of early rice under different treatments %
% 处理
Treatment茎鞘比例Ratio of stem-sheath 叶片比例Ratio of leaf 穗比例Ratio of panicle 抽穗期
Heading stage灌浆期
Filling stage成熟期
Maturity stage抽穗期
Heading stage灌浆期
Filling stage成熟期
Maturity stage抽穗期
Heading stage灌浆期
Filling stage成熟期
Maturity stageA 38.67±0.33c 28.00±0.58a 22.00±0.58a 41.33±0.33a 18.00±0.00a 11.67±0.67bc 20.00±0.58ab 54.00±0.58a 66.33±0.33bc B 43.33±0.67ab 27.67±1.67a 20.67±1.20a 36.67±0.33c 21.66±2.67a 10.67±0.33c 20.00±1.00ab 50.67±4.33a 68.67±0.89a C 46.50±1.44a 26.25±1.25a 20.75±0.75a 35.00±0.41d 17.75±0.75a 13.25±0.48ab 18.50±1.71b 56.00±1.87a 66.00±0.71bc D 43.50±0.50ab 26.50±0.50a 21.50±0.50a 33.00±0.00e 19.00±0.00a 14.00±0.00a 23.50±0.50a 54.50±0.50a 64.50±0.50c E 41.75±0.75bc 28.50±0.87a 20.25±0.25a 38.25±0.63b 18.50±0.29a 12.00±0.41bc 20.00±1.29ab 53.00±1.00a 67.75±0.25ab A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level.
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表 4 各处理早稻干物质的转运
Table 4 Dry matter transformation of early rice under different treatments
处理
Treatment茎叶干物质输出量
Dry matter exportation from in stem-sheath and leaf (t·hm-2)茎叶干物质输出率
Output percent of dry matter fromin stem-sheath and leaf (%)表观转变率
Apparent conversion rate (%)A 0.11±0.01c 3.31±0.07c 2.20±0.06c B 0.54±0.01a 15.17±0.43a 9.41±0.08a C 0.41±0.02b 10.79±0.43b 7.17±0.10b D 0.12±0.01c 3.46±0.08c 2.39±0.12c E 0.09±0.01c 2.98±0.10c 1.66±0.07d A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+ 120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level.
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表 5 各处理早稻成熟期茎、叶、穗的氮素养分含量及吸收量
Table 5 Nitrogen contents and nutrient uptakes of stem-sheath, leaf, panicle in at maturity stages of early rice under different treatments
处理
Treatment全氮含量Total nitrogen content (%) 氮素养分吸收量Nitrogenutrient uptakes (kg·hm-2) 茎Stem-sheath 叶Leaf 穗Panicle 茎Stem-sheath 叶Leaf 穗Panicle A 1.14±0.13ab 2.08±0.15a 1.45±0.04bc 21.67±1.97ab 21.03±1.24ab 83.60±1.49b B 1.01±0.04b 1.83±0.15a 1.45±0.00bc 19.96±0.93b 19.16±1.70b 95.96±2.20a C 1.36±0.03a 1.81±0.07a 1.41±0.02c 33.48±5.64a 23.73±1.58ab 92.68±2.77a D 1.34±0.11a 1.87±0.02a 1.50±0.03b 27.52±3.63ab 24.29±0.58a 91.33±4.02a E 1.43±0.10a 2.03±0.06a 1.57±0.01a 26.61±1.99ab 21.92±0.73ab 96.80±1.05a A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level.
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表 6 各处理早稻的氮素干物质生产效率 (NDMPE) 和氮收获指数 (NHI)
Table 6 Nitrogen dry matter production efficiency (NDMPE) and nitrogen harvest index (NHI) of early rice under different treatments
处理Treatment NDMPE (kg·kg-1) NHI (%) A 68.73±4.31a 66.19±1.08bc B 71.44±3.08a 71.04±1.45a C 66.52±2.87a 61.83±1.35c D 65.95±7.97a 63.80±1.43bc E 62.62±1.84a 66.61±1.08b A:冬闲+150 kg (N)·hm-2; B:紫云英+90 kg (N)·hm-2; C:紫云英+ 120 kg (N)·hm-2; D:紫云英+150 kg (N)·hm-2; E:紫云英+180 kg (N)·hm-2。数据为3个重复的平均值±标准误; 同列不同小写字母分别表示各处理间差异显著 (P < 0.05)。A: winter fallow with 150 kg (N)·hm-2 application in early rice; B: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; C: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; D: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice; E: Chinese milk vetch plantation in winter and incorporation in spring combined with 90 kg (N)·hm-2 application in early rice. Values in the table are mean±SE of 3 replicates. Different lowercase letters in the same column mean significant differences at 0.05 level.
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