Abstract: Carbon footprint is the CO₂ equivalent emissions directly or indirectly emitted from the production of a product over its life cycle. In promoting green and sustainable development, the carbon footprint of a product is an important indicator of its environmental impact, guiding enterprises and individuals to formulate corresponding emission reduction measures and green development strategies. China is the origin of Myrica rubra, with a history of cultivation and utilization dating back at least 2 000 years. Myrica rubra has been widely cultivated and produced in several regions and has become a local agricultural specialty and a leading industry. This study examined the carbon footprint of fresh Myrica rubra fruit from Huaihua, China, using the life cycle assessment method (LCA). The accounting procedure used ISO 14067 and PAS 2050 carbon footprint accounting techniques. Because Myrica rubra fruit lacked the designated carbon footprint of the product-product category rule (CFP-PCR), this study defined the functional unit as producing 1 kg fresh Myrica rubra fruit based on real-world scenarios. System boundary of this study was from cradle to farm gate. The raw material procurement, planting process (weeding, shaping, pruning, fertilization, and picking), and packaging and storage were covered by the activity level research data, and these data were collected through on-site inquiries. The emission factors were obtained from the IPCC, Chinese Life Cycle Basic Database (CLCD), China Products Carbon Footprint Factors Database (CPCD 2.0), and the published literatures. The product carbon footprint of fresh Myrica rubra fruit in the plantation base was 0.185 kg(CO₂-eq)·kg⁻¹, of which the contribution of raw material procurement, planting process, and packaging and storage to the overall carbon footprint were 63.00%, 36.31%, and 0.69%, respectively. Comparing the different carbon emission sources, upstream fertilizer production and direct emissions after fertilizer application were the most important carbon emission sources for the carbon footprint of fresh Myrica rubra fruit, and the cumulative contribution reached 91.00%, of which compound fertilizer was 59.91% and organic fertilizer was 31.09%. Analyzing the individual carbon emission sources, upstream compound fertilizer production contributed the most to the carbon footprint of the product (42.92%), followed by upstream organic fertilizer production (18.56%), therefore the reduction and efficiency of compound fertilizer is significant to the carbon emission reduction of fresh Myrica rubra fruit and is the key link in carbon emission reduction. Uncertainty analysis (assessing the reliability of the data and calculation process) and sensitivity analysis were conducted using the methods specified in ISO 14067. The uncertainty of the carbon footprint accounting results in this study was small (the variation of the contribution rate was ±9%). When the activity data of the compound fertilizer and organic fertilizer varied from −20% to 20%, the sensitivity of compound fertilizer was higher (the variation of the contribution rate was from −8.28% to 7.24%). Sensitivity of organic fertilizer was lower, and the contribution rate ranged from −5.39% to 5.04%). Without affecting product functionality and efficiency, fertilizers (e.g. organic fertilizer, slow released fertlizer and biochar), and bio-based and biodegradable packaging materials with lower carbon emission factors, as well as cleaner transportation (e.g. electric) and energy supply (e.g. photovoltaic) are recommonded. Ultimately, the aim is to help achieve local carbon peak and carbon neutrality targets by providing a reference for the scientific management of greenhouse gas emissions in the Myrica rubra sector and promoting the advancement of low-carbon agriculture.