盐胁迫下赤霉素浸种对紫花苜蓿的缓解效应研究

The alleviating effect of gibberellin seed soaking on salt stress in Alfalfa

  • 摘要: 紫花苜蓿 (Medicago sativa L.) 的种植区多在我国东北、华北、西北等土地盐渍化严重的地区, 这导致紫花苜蓿易受到盐胁迫的危害, 造成产量损失。因此, 本试验探究了在盐 (Nacl) 胁迫下, 外源赤霉素 (GA) 浸种对紫花苜蓿的缓解作用以及赤霉素最佳浸种浓度与盐胁迫程度之间的关系。实验设置0.3%、0.6%和0.9%三种盐胁迫程度, 以及50、100、150mg/L三种浸种浓度。测定了紫花苜蓿幼苗的生物量, 抗氧化酶活性、离子含量以及激素含量等指标。结果表明, 适宜浓度的赤霉素浸种通过提高盐胁迫下苜蓿幼苗的抗氧化酶活性、钾离子、生长素、水杨酸含量以及降低脱落酸、氯离子、钠离子含量等途径来缓解盐胁迫。且赤霉素的最佳施用浓度与盐胁迫程度之间呈反比关系: 在0.3%低盐胁迫下100-150mg/L的赤霉素浸种效果较好, 在0.6%盐胁迫下100mg/L左右的赤霉素浸种效果较好, 在0.9%高浓度盐胁迫下50mg/L左右的赤霉素浸种效果较好。本研究证明, 外源赤霉素浸种可以缓解盐胁迫对苜蓿幼苗的毒害, 促进其生长, 且在低中度盐胁迫下施加效果要优于重度盐胁迫。这些发现为提高盐碱地区紫花苜蓿的种植效益提供了理论依据和实践指导。

     

    Abstract: Alfalfa (Medicago sativa L.) is primarily cultivated in regions of Northeast, North, and Northwest China where soil salinization is severe, making it susceptible to salt stress and resulting in yield losses. This study investigated the alleviating effects of exogenous gibberellin (GA) seed soaking on alfalfa under salt stress and the relationship between optimal GA soaking concentration and salt stress levels. The experiment employed three salt stress levels (0.3%, 0.6%, and 0.9%) and three GA soaking concentrations (50, 100, and 150 mg/L). Biomass, antioxidant enzyme activity, ion content, and hormone levels in alfalfa seedlings were measured. Results showed that appropriate GA concentrations alleviated salt stress by increasing antioxidant enzyme activity, potassium ions, auxins, and salicylic acid content, while reducing abscisic acid, chloride ions, and sodium ions in alfalfa seedlings under salt stress. The optimal GA concentration was inversely proportional to salt stress intensity: 100-150 mg/L GA was most effective under 0.3% salt stress, approximately 100 mg/L under 0.6% salt stress, and around 50 mg/L under 0.9% salt stress. High concentrations of both salt and GA exhibited synergistic inhibitory effects on alfalfa seedlings. This study demonstrates that exogenous GA seed soaking can mitigate salt stress toxicity in alfalfa seedlings, promote their growth, and is more effective under low to moderate salt stress compared to severe salt stress. These findings provide theoretical basis and practical guidance for improving alfalfa cultivation in salt-affected regions.

     

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